*The first two authors contributed equally to this paper.
Correlation of hepatitis B virus (HBV) genotypes and mutations in basal core promoter/precore with clinical features of chronic HBV infection
Article first published online: 19 FEB 2007
Volume 27, Issue 2, pages 240–246, March 2007
How to Cite
Du, H., Li, T., Zhang, H.-Y., He, Z.-P., Dong, Q.-M., Duan, X.-Z. and Zhuang, H. (2007), Correlation of hepatitis B virus (HBV) genotypes and mutations in basal core promoter/precore with clinical features of chronic HBV infection. Liver International, 27: 240–246. doi: 10.1111/j.1478-3231.2006.01400.x
- Issue published online: 19 FEB 2007
- Article first published online: 19 FEB 2007
- Received 2 June 2006accepted 16 October 2006
- chronic HBV infection;
- hepatitis B virus (HBV);
- hepatitis e antigen (HBeAg);
Background/Aims: To investigate the correlation of hepatitis B virus (HBV) genotypes and basal core promoter (BCP) and precore (PC) mutations in patients with chronic hepatitis B.
Methods: HBV genotyping, nucleotide mutation, serum HBV DNA level and serological markers were analyzed in 121 patients with chronic HBV infection using INNO-LiPA HBV genotyping, polymerase chain reaction (PCR) product-based sequencing, fluorescence quantitative PCR and enzyme-linked immunosorbent assays respectively.
Results: Forty (33.0%), 77 (63.6%), two (1.7%) and two (1.7%) patients had genotypes B, C, B/C and D infections respectively. Significant differences were found in serum HBV DNA levels (log10 copies/ml: 6.18 vs. 5.61, P=0.042) and mutations at nucleotide (nt) 1762/1764 (71.4% vs. 42.5%, P=0.002) between genotypes C- and B-infected patients. There were significant differences in the mean age, serum biochemical parameter levels and mutation rates in BCP/PC among hepatitis e antigen (HBeAg)-positive and -negative chronic hepatitis B (CHB) and liver cirrhosis (LC) groups.
Conclusion: Genotypes C and B are predominant in China, and the frequent nt 1762/1764 mutation, which occurs commonly in HBeAg-negative CHB, especially in genotype C patients, may be associated with the progress of chronic HBV infection.