Timing of ischaemia/reperfusion before hepatectomy without inflow occlusion determines liver damage in rats: role of heat shock protein 70
Article first published online: 27 SEP 2007
Volume 27, Issue 10, pages 1323–1332, December 2007
How to Cite
Nakamura, I., Isaji, S., Yamagiwa, K., Hamada, T. and Uemoto, S. (2007), Timing of ischaemia/reperfusion before hepatectomy without inflow occlusion determines liver damage in rats: role of heat shock protein 70. Liver International, 27: 1323–1332. doi: 10.1111/j.1478-3231.2007.01575.x
- Issue published online: 27 SEP 2007
- Article first published online: 27 SEP 2007
- Received 15 May 2007accepted 30 July 2007
- liver regeneration;
Background: Living donor hepatectomies in liver transplantation are usually performed without inflow occlusion. We hypothesized that selective ischaemia/reperfusion (SIR) before partial hepatectomy (PH) without inflow occlusion might exert a hepatoprotective effect.
Methods: In the SIR groups, rats were subjected to a selective 30-min ischaemia to the liver that remained after PH, followed by various durations of reperfusion before 70% PH without inflow occlusion. The control group underwent 70% PH alone.
Results: As assessed by serum aspartate and alanine aminotransferase levels, 30-min reperfusion was highly protective against liver injury compared with 10-min reperfusion, showing the same levels as that of the control group. After PH in the 10-min reperfusion group, apoptotic cells were significantly higher and the 7-day survival rate was significantly lower than that of the 30-min reperfusion group and the control group. In the 30-min reperfusion group, the expression of heat shock protein 70 (HSP70) was significantly higher than that in the 10-min reperfusion group, while apoptosis was improved to the levels of the control group. In the SIR groups, liver regeneration was significantly enhanced, with markedly increased levels of interleukin 6 (IL-6) compared with the control group.
Conclusions: The timing of SIR before PH without inflow occlusion seemed to be the most important factor for determining liver damage and survival in the context of HSP70 production, while high levels of IL-6 appear to be associated with liver regeneration after PH. The procedure of SIR before PH is not recommended because the SIR groups did not overcome the control group.