Hepatic stellate cell line modulates lipogenic transcription factors
Article first published online: 3 OCT 2007
Volume 27, Issue 9, pages 1255–1264, November 2007
How to Cite
Guimarães, E. L. M., Franceschi, M. F. S., Andrade, C. M. B., Guaragna, R. M., Borojevic, R., Margis, R., Bernard, E. A. and Guma, F. C. R. (2007), Hepatic stellate cell line modulates lipogenic transcription factors. Liver International, 27: 1255–1264. doi: 10.1111/j.1478-3231.2007.01578.x
- Issue published online: 3 OCT 2007
- Article first published online: 3 OCT 2007
- Received 26 December 2006accepted 26 June 2007
- hepatic stellate cell;
Background/Aims: Pre-adipocyte differentiation into adipocyte is a terminal differentiation process triggered by a cascade of transcription factors. Conversely, hepatic stellate cells (HSC) can switch between lipid storing and the myofibroblast phenotype in association with liver fibrotic processes. Here, adipogenic/lipogenic-related transcription factors and downstream-regulated genes were evaluated in a murine HSC cell line. GRX-HSC cells are transitional myofibroblasts that differentiate into lipocytes following retinol or indomethacin treatment.
Methods/Results: Specific mRNAs were quantified by a real-time polymerase chain reaction after 24 h or 7 days of cell culture with indomethacin or retinol. Proliferator-activated receptorγ and Pex16 transcripts were increased either by retinol or indomethacin. Retinol induced a minor increase in C/enhancer binding proteinα transcripts, while only indomethacin increased adipsin transcripts.
Conclusions: Our results showed that the myofibroblast to lipocyte phenotype switch follows partially different transcriptional pathways, according to the effector. Retinol induces lipid synthesis and storage without affecting characteristic adipocytic genes, while indomethacin treatment restores the lipocytic phenotype with increased adipisin expression.