Overexpression of NK2 promotes liver fibrosis in carbon tetrachloride-induced chronic liver injury
Article first published online: 1 NOV 2007
Volume 28, Issue 1, pages 126–131, January 2008
How to Cite
Hagiwara, S., Otsuka, T., Yamazaki, Y., Kosone, T., Sohara, N., Ichikawa, T., Sato, K., Kakizaki, S., Takagi, H. and Mori, M. (2008), Overexpression of NK2 promotes liver fibrosis in carbon tetrachloride-induced chronic liver injury. Liver International, 28: 126–131. doi: 10.1111/j.1478-3231.2007.01616.x
- Issue published online: 1 NOV 2007
- Article first published online: 1 NOV 2007
- Received 28 April 2007accepted 19 September 2007
Vol. 28, Issue 4, 582, Article first published online: 12 MAR 2008
- hepatocyte growth factor;
- liver fibrosis;
Background/Aims: Hepatocyte growth factor (HGF) inhibits liver fibrosis induced by carbon tetrachloride (CCl4) in animal models. NK2 is a natural splice variant of HGF, but its in vivo function remains to be elucidated. We investigated the in vivo effects of NK2 on CCl4-induced liver fibrosis.
Methods: NK2 transgenic mice and wild-type (WT) mice were injected intraperitoneally with CCl4 twice a week. The extent of hepatic fibrosis was evaluated by Azan–Mallory staining. Expression levels of mRNAs of transforming growth factor-β1 (TGF-β1) and matrix metalloproteinase-13 (MMP-13) were examined by real-time polymerase chain reaction. The protein levels of α-smooth muscle actin (α-SMA), c-Met and its phosphorylation were determined by Western blot analysis.
Results: Liver fibrosis was significantly more severe in NK2 transgenic mice than in WT mice. CCl4 administration increased the expression levels of TGF-β1 mRNA and α-SMA protein, and decreased the expression of MMP-13 mRNA in livers of NK2 transgenic mice compared with those of WT mice. c-Met protein expression in the liver was compatible with the degree of fibrosis. As for c-Met activation, no difference was found between NK2 and WT livers.
Conclusion: Overexpression of NK2 acts as an antagonist of HGF and promotes liver fibrosis in CCl4-induced chronic liver injury.