Viral suppression correlates with dendritic cell restoration in chronic hepatitis B patients with autologous cytokine-induced killer cell transfusion
Article first published online: 15 AUG 2008
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd
Volume 29, Issue 3, pages 466–474, March 2009
How to Cite
Shi, M., Fu, J., Shi, F., Zhang, B., Tang, Z., Zhang, Z., Zhang, H., Jin, L., Chen, L., Wang, H., Akbar, S. M. F. and Wang, F.-S. (2009), Viral suppression correlates with dendritic cell restoration in chronic hepatitis B patients with autologous cytokine-induced killer cell transfusion. Liver International, 29: 466–474. doi: 10.1111/j.1478-3231.2008.01861.x
- Issue published online: 4 FEB 2009
- Article first published online: 15 AUG 2008
- Received 6 May 2008Accepted 14 July 2008
- adoptive therapy;
- chronic hepatitis B;
- cytokine-induced killer cells;
- hepatitis B virus;
- myeloid dendritic cells;
- plasmacytoid dendritic cells
Background: Myeloid and plasmacytoid dendritic cells (mDCs, pDCs) are functionally impaired in patients with chronic hepatitis B (CHB). Adoptive immunotherapy can suppress hepatitis B virus (HBV) replication in CHB patients, but whether it can restore the functionality of mDCs and pDCs remains unknown.
Methods: Autologous cytokine-induced killer (CIK) cells obtained from 14 CHB patients were transfused back to patients, case by case, to observe the effect of CIK-cell treatment on the frequency and functionality of mDCs and pDCs in CHB patients during a 24-week follow-up investigation.
Results: Seven virological responders exhibited a sustained decrease in HBV load after CIK-cell transfusion; another seven non-virological responders showed only sustained high levels of HBV load during the 24-week period following CIK-cell transfusion. The rate of hepatitis B e antigen loss or seroconversion was also higher in virological responders than in non-virological responders. Importantly, we found that the frequency and cytokine-producing capacity of mDCs and pDCs increased significantly in virological responders, but not in non-virological responders. In addition, these patients exhibited a close correlation between restoration DC subsets and a decrease in HBV DNA load, rather than a change in the alanine aminotransferase level.
Conclusion: Cytokine-induced killer-cell treatment reduced HBV DNA load in some CHB patients; the efficiency at least partially correlates with the restoration of frequency and functionality of mDCs and pDCs.