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Fluorescence spectroscopy to diagnose hepatic steatosis in a rat model of fatty liver

Authors

  • Gustavo Ribeiro De Oliveira,

    1. Faculty of Medicine of Ribeirão Preto, Institute of Physics of São Carlos (IFSC), University of São Paulo (USP), São Carlos, SP, Brazil
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  • Ajith K. Sankarankutty,

    1. Faculty of Medicine of Ribeirão Preto, Institute of Physics of São Carlos (IFSC), University of São Paulo (USP), São Carlos, SP, Brazil
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  • Orlando Castro e Silva,

    1. Faculty of Medicine of Ribeirão Preto, Institute of Physics of São Carlos (IFSC), University of São Paulo (USP), São Carlos, SP, Brazil
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  • Juliana Ferreira,

    1. Faculty of Medicine of Ribeirão Preto, Institute of Physics of São Carlos (IFSC), University of São Paulo (USP), São Carlos, SP, Brazil
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  • Cristina Kurachi,

    1. Physics Institute of São Carlos (IFSC), University of São Paulo (USP), São Carlos, SP, Brazil
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  • Sergio Zucoloto,

    1. Faculty of Medicine of Ribeirão Preto, Institute of Physics of São Carlos (IFSC), University of São Paulo (USP), São Carlos, SP, Brazil
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  • Hélio Vannucchi,

    1. Faculty of Medicine of Ribeirão Preto, Institute of Physics of São Carlos (IFSC), University of São Paulo (USP), São Carlos, SP, Brazil
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  • Alceu Afonso Jordão Jr,

    1. Faculty of Medicine of Ribeirão Preto, Institute of Physics of São Carlos (IFSC), University of São Paulo (USP), São Carlos, SP, Brazil
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  • Júlio Sergio Marchini,

    1. Faculty of Medicine of Ribeirão Preto, Institute of Physics of São Carlos (IFSC), University of São Paulo (USP), São Carlos, SP, Brazil
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  • Vanderlei Salvador Bagnato

    1. Physics Institute of São Carlos (IFSC), University of São Paulo (USP), São Carlos, SP, Brazil
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Correspondence
Dra. Juliana Ferreira, Faculty of Medicine of Ribeirão Preto, Institute of Physics of São Carlos (IFSC), University of São Paulo (USP), Avenue Trabalhador São Carlense, 400 Cx. Postal 369, CEP: 13560-970, São Carlos, SP, Brazil
Tel: +55 16 3373 9829
Fax: +55 16 3373 9811
e-mail: juferreira@if.sc.usp.br

Abstract

Background: Steatosis is diagnosed on the basis of the macroscopic aspect of the liver evaluated by the surgeon at the time of organ extraction or by means of a frozen biopsy.

Aim: In the present study, the applicability of laser-induced fluorescence (LIF) spectroscopy was investigated as a method for the diagnosis of different degrees of steatosis experimentally induced in rats.

Material and methods: Rats received a high-lipid diet for different periods of time. The animals were divided into groups according to the degree of induced steatosis diagnosis by histology. The concentration of fat in the liver was correlated with LIF by means of the steatosis fluorescence factor (SFF).

Results: The histology classification, according to liver fat concentration was, Severe Steatosis, Moderate Steatosis, Mild Steatosis and Control (no liver steatosis). Fluorescence intensity could be directly correlated with fat content. It was possible to estimate an average of fluorescence intensity variable by means of different confidence intervals (P=95%) for each steatosis group. SFF was significantly higher in the Severe Steatosis group (P<0.001) compared with the Moderate Steatosis, Mild Steatosis and Control groups.

Conclusion: The various degrees of steatosis could be directly correlated with SFF. LIF spectroscopy proved to be a method capable of identifying the degree of hepatic steatosis in this animal model, and has the potential of clinical application for non-invasive evaluation of the degree of steatosis.

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