Survivin is upregulated during liver regeneration in rats and humans and is associated with hepatocyte proliferation
Article first published online: 3 NOV 2008
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd
Volume 29, Issue 4, pages 585–592, April 2009
How to Cite
Baba, H. A., Wohlschlaeger, J., Schmitz, K. J., Nadalin, S., Lang, H., Benesch, A., Gu, Y., Biglarnia, A.-R., Sotiropoulos, G. C., Takeda, A., Takeda, N., Von Wnuck Lipinski, K. and Levkau, B. (2009), Survivin is upregulated during liver regeneration in rats and humans and is associated with hepatocyte proliferation. Liver International, 29: 585–592. doi: 10.1111/j.1478-3231.2008.01911.x
- Issue published online: 10 MAR 2009
- Article first published online: 3 NOV 2008
- Received 8 May 2008Accepted 12 September 2008
- liver regeneration;
- living donor liver transplantation;
Background: Survivin regulates cell division and inhibits apoptosis. Liver regeneration is a complex process involving both proliferation and apoptosis. The role of survivin is not well elucidated and no data exist in humans.
Methods: Seventy per cent liver resection was used to investigate liver regeneration in rats. Survivin was identified by means of reverse transcriptase polymerase chain reaction, Western blotting and immunohistochemistry. Proliferation and apoptosis were quantified. Liver biopsies from 33 patients who underwent living donor liver transplantation were used to study survivin immuno-expression, proliferation and apoptosis within the first 17 days after transplantation. Seven healthy donors served as controls.
Results: Survivin transcript and protein were significantly upregulated in rat hepatocytes after 24–72 h during regeneration and showed a significant correlation with proliferation but not with apoptosis. In humans, survivin was nearly absent in donor and reperfused liver tissue but increased significantly 5–7 days after transplantation and correlated with proliferation but not with apoptosis.
Conclusions: Survivin is upregulated in human and rodent liver regeneration and correlates with proliferation, suggesting an association of survivin and cell division.