dUTP pyrophosphatase expression correlates with a poor prognosis in hepatocellular carcinoma
Article first published online: 30 NOV 2009
© 2009 John Wiley & Sons A/S
Volume 30, Issue 3, pages 438–446, March 2010
How to Cite
Takatori, H., Yamashita, T., Honda, M., Nishino, R., Arai, K., Yamashita, T., Takamura, H., Ohta, T., Zen, Y. and Kaneko, S. (2010), dUTP pyrophosphatase expression correlates with a poor prognosis in hepatocellular carcinoma. Liver International, 30: 438–446. doi: 10.1111/j.1478-3231.2009.02177.x
- Issue published online: 18 JAN 2010
- Article first published online: 30 NOV 2009
- Received 13 August 2009Accepted 26 October 2009
Fig. S1. Subcellular localization of genes detected in each SAGE library.
Fig. S2. Microarray analysis of DUT and TS gene expression in 238 HCC cases publicly available (GSE5975). DUT was overexpressed more than 2-fold in 121 of 238 HCC tissues (median: 2.03), whereas TS was overexpressed more than 2-fold in 54 of 238 HCC tissues (median: 1.41) compared with the non-cancerous liver tissues.
Fig. S3. (A) Transfection of siRNAs targeting DUT (DUT2) decreased DUT expression compared with the control (scrambled sequence). Gene expression was evaluated in triplicates 72 hours after transfection (mean ± SD). (B) DUT gene knockdown sensitized HuH7 cells to low-dose 5-FU (0.25 mg/ml) (mean ± SD).
Fig. S4. Nuclear and cytoplasmic dUTPase expression and cell proliferation in HCC. PCNA indexes in nuclear dUTPase-high HCC were higher than those in -low HCC with statistical significance (P = 0.01). Cytoplasmic dUTPase expression was not associated with PCNA indexes in HCC.
Table S1. A summary of constructed SAGE libraries.
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