Increased PD-1 and decreased CD28 expression in chronic hepatitis B patients with advanced hepatocellular carcinoma
Article first published online: 25 AUG 2010
© 2010 John Wiley & Sons A/S
Volume 30, Issue 9, pages 1379–1386, October 2010
How to Cite
Hsu, P.-N., Yang, T.-C., Kao, J.-T., Cheng, K.-S., Lee, Y.-J., Wang, Y.-M., Hsieh, C.-T., Lin, C.-W. and Wu, Y.-Y. (2010), Increased PD-1 and decreased CD28 expression in chronic hepatitis B patients with advanced hepatocellular carcinoma. Liver International, 30: 1379–1386. doi: 10.1111/j.1478-3231.2010.02323.x
- Issue published online: 1 SEP 2010
- Article first published online: 25 AUG 2010
- Received 1 April 2010Accepted 19 July 2010
- hepatocellular carcinoma;
- tumour-infiltrating lymphocytes
Background/aims: Hepatitis B infection is a well-known cause of hepatocellular carcinoma (HCC). This study aims to investigate the role that the co-stimulatory molecule CD28 and co-inhibitory molecule programmed death-1 (PD-1) play in compromising the function of tumour-infiltrating lymphocytes (TIL) in hepatitis B virus (HBV)-related HCC.
Methods: A total of 45 patients with HBV-related HCC were enrolled during the period February 2008 to March 2010. The immune phenotype and the expression of PD-1, CD28 and CD127 in TIL in biopsy specimens and in peripheral blood lymphocytes (PBL) from the same patients were analysed by flow cytometry.
Results: Among the 45 patients, there was a male predominance (80%) and the mean age was 50 ± 13.68 years (range: 29–71). The majority of TIL were CD45RO+CD69+. PD-1 expression was higher and CD28 and CD127 expression levels were lower in TIL than in PBL. The prevalence of portal vein thrombosis was 40%. Furthermore, tumour thrombosis invasion into the portal vein correlated with the expression level of the PD-1 co-inhibitory molecule.
Conclusion: PD-1+ tumour-infiltrating lymphocytes correlate with portal vein thrombosis and might serve as a potential prognostic marker of and a novel therapeutic target for HBV-related HCC.