Expression of common housekeeping genes is affected by disease in human hepatitis C virus-infected liver
Article first published online: 15 NOV 2010
© 2010 John Wiley & Sons A/S
Volume 31, Issue 3, pages 386–390, March 2011
How to Cite
Congiu, M., Slavin, J. L. and Desmond, P. V. (2011), Expression of common housekeeping genes is affected by disease in human hepatitis C virus-infected liver. Liver International, 31: 386–390. doi: 10.1111/j.1478-3231.2010.02374.x
- Issue published online: 1 FEB 2011
- Article first published online: 15 NOV 2010
- Received 10 July 2010, Accepted 25 October 2010
- housekeeping genes;
- liver disease
Background: Comparative gene expression is commonly determined with reference to the expression of a housekeeping gene (HKG), the level of which is assumed to be unregulated. There are little data to date on the effect of disease on the expression of classic HKGs in hepatitis C virus (HCV)-infected human liver.
Aims: To identity HKGs stable across a wide spectrum of disease in human HCV-infected liver.
Methods: β-Actin, hypoxanthine phosphoribosyltransferase 1 (HPRT1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), splicing factor arginine/serine-rich 4, β-glucuronidase and 18S ribosomal RNA (18S rRNA) were measured by real-time polymerase chain reaction in liver biopsy tissue. Samples were categorised for inflammation, fibrosis and steatosis, and allocated into groups with mild or severe liver disease. Values were analysed using Spearman's rank correlation, NormFinder, BestKeeper and geNorm programs.
Results: All genes performed well in the samples of patients with low disease activity, but HPRT1, β-actin, GAPDH and 18S rRNA ranked poorly in samples with severe fibrosis or inflammation.
Conclusions: Our results indicate that liver disease affects the expression of common HKGs and that β-glucuronidase and splicing factor arginine/serine-rich 4 are the most stable HKGs from this group for studies of gene expression in HCV-infected human liver.