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How to use virological tools for the optimal management of chronic hepatitis C
Article first published online: 4 JAN 2011
DOI: 10.1111/j.1478-3231.2010.02398.x
© 2011 John Wiley & Sons A/S
Issue

Liver International
Special Issue: Proceedings of the 4th Paris Hepatitis Conference. The publication of this supplement was supported by an unrestricted educational grant from F. Hoffmann-Laroche Ltd.
Volume 31, Issue Supplement s1, pages 3–12, January 2011
Additional Information
How to Cite
de Leuw, P., Sarrazin, C. and Zeuzem, S. (2011), How to use virological tools for the optimal management of chronic hepatitis C. Liver International, 31: 3–12. doi: 10.1111/j.1478-3231.2010.02398.x
Publication History
- Issue published online: 4 JAN 2011
- Article first published online: 4 JAN 2011
- Received 10 November 2010Accepted 26 November 2010
References
- 1The global burden of hepatitis C. Liver Int 2009; 29 (Suppl. 1): 74–81.
- 2, , The changing epidemiology of hepatitis C virus infection in Europe. J Hepatol 2008; 48: 148–62.
- 3, , , et al. Molecular biology of the hepatitis C viruses: implications for diagnosis, development and control of viral disease. Hepatology 1991; 14: 381–8.
- 4, , , et al. Production of infectious hepatitis C virus in tissue culture from a cloned viral genome. Nat Med 2005; 11: 791–6.
- 5, , Replication of hepatitis C virus. Nat Rev Microbiol 2007; 5: 453–63.
- 6, , , et al. Persistence of viremia and the importance of long-term follow-up after acute hepatitis C infection. Hepatology 1999; 29: 908–14.
- 7, , , et al. Acute hepatitis C: high rate of both spontaneous and treatment-induced viral clearance. Gastroenterology 2003; 125: 80–8.
- 8, , Acute hepatitis C. Hepatology 2001; 33: 321–7.
- 9, , , et al. Sustained virologic response and clinical outcomes in patients with chronic hepatitis C and advanced fibrosis. Ann Intern Med 2007; 147: 677–84.
- 10, , , et al. Outcome of sustained virological responders with histologically advanced chronic hepatitis C. Hepatology 2010; 52: 833–44.
- 11, , , et al. Expert opinion on the treatment of patients with chronic hepatitis C. J Viral Hepat 2009; 16: 75–90.
- 12, , , et al. Peginterferon alfa-2b plus ribavirin compared with interferon alfa-2b plus ribavirin for initial treatment of chronic hepatitis C: a randomised trial. Lancet. 2001; 358: 958–65.
- 13, , , et al. Peginterferon alfa-2a plus ribavirin for chronic hepatitis C virus infection. N Engl J Med 2002; 347: 975–82.
- 14, , IDEAL Study Team et al. Peginterferon alfa-2b or alfa-2a with ribavirin for treatment of hepatitis C infection. N Engl J Med 2009; 361: 580–93.
- 15, , HEPATITIS Group et al. Sensitivity and specificity of third-generation hepatitis C virus antibody detection assays: an analysis of the literature. J Viral Hepat 2001; 8: 87–95.
- 16, Evaluation of a third generation anti-HCV assay in predicting viremia in patients with positive HCV antibodies. Ann Hepatol 2002; 1: 179–82.
- 17, , , , et al. Multicenter evaluation of a fully automated third-generation anti-HCV antibody screening test with excellent sensitivity and specificity. Med Microbiol Immunol 24 September 2010 [Epub ahead of print] DOI: DOI: 10.1007/s00430-010-0171-0.
- 18, , , et al. Evaluation of third-generation screening and confirmatory assays for HCV antibodies. Vox Sang 1994; 66: 122–9.
- 19, , , et al. Prospective multicenter clinical evaluation of AMPLICOR and COBAS AMPLICOR hepatitis C virus tests. J Clin Microbiol 2001; 39: 4005–12.
- 20, , , et al. Improved version 2.0 qualitative and quantitative AMPLICOR reverse transcription-PCR tests for hepatitis C virus RNA: calibration to international units, enhanced genotype reactivity, and performance characteristics. J Clin Microbiol 2000; 38: 4171–9.
- 21, , , et al. Performance of the COBAS AMPLICOR HCV MONITOR test, version 2.0, an automated reverse transcription-PCR quantitative system for hepatitis C virus load determination. J Clin Microbiol 2000; 38: 2210–4.
- 22, , , et al. Quantitation of hepatitis C virus RNA by third generation branched DNA-based signal amplification assay. J Virol Methods 2002; 101: 159–68.
- 23, , , et al. Detection of residual hepatitis C virus RNA by transcription-mediated amplification in patients with complete virologic response according to polymerase chain reaction-based assays. Hepatology 2000; 32: 818–23.
- 24, , , et al. Performance attributes of the LCx HCV RNA quantitative assay. J Virol Methods 2004; 115: 207–15.
- 25, , , et al. Multicenter evaluation of the VERSANT HCV RNA qualitative assay for detection of hepatitis C virus RNA. J Clin Microbiol 2003; 41: 651–6.
- 26, , , et al. Differences between two real-time PCR-based hepatitis C virus (HCV) assays (RealTime HCV and Cobas AmpliPrep/Cobas TaqMan) and one signal amplification assay (Versant HCV RNA 3.0) for RNA detection and quantification. J Clin Microbiol 2008; 46: 388–91.
- 27, , , et al. Evaluation of the analytical performance of the new Abbott RealTime RT-PCRs for the quantitative detection of HCV and HIV-1 RNA. J Clin Virol 2007; 40: 99–104.
- 28, , , et al. Evaluation of an automated, highly sensitive, real-time PCR-based assay (COBAS Ampliprep/COBAS TaqMan) for quantification of HCV RNA. J Clin Virol 2008; 43: 162–8.
- 29, , , et al. Fully automated quantification of hepatitis C virus (HCV) RNA in human plasma and human serum by the COBAS AmpliPrep/COBAS TaqMan system. J Clin Virol 2007; 38: 326–33.
- 30, , , et al. Comparison of conventional PCR with real-time PCR and branched DNA-based assays for hepatitis C virus RNA quantification and clinical significance for genotypes 1 to 5. J Clin Microbiol 2006; 44: 729–37.
- 31, , , et al. Overestimation and underestimation of hepatitis C virus RNA levels in a widely used real-time polymerase chain reaction-based method. Hepatology 2007; 46: 22–31.
- 32, , Identification of a highly conserved sequence element at the 3′ terminus of hepatitis C virus genome RNA. J Virol 1996; 70: 3363–71.
- 33, , , et al. Structure of the 3′ terminus of the hepatitis C virus genome. J Virol 1996; 70: 3307–12.
- 34Genetic diversity and evolution of hepatitis C virus – 15 years on. J Gen Virol 2004; 85: 3173–88.
- 35, , , et al. Consensus proposals for a unified system of nomenclature of hepatitis C virus genotypes. Hepatology 2005; 42: 962–73.
- 36, , , et al. Automated sample preparation for the TRUGENE HIV-1 genotyping kit using the MagNA pure LC instrument. Diagn Microbiol Infect Dis 2004; 49: 59–61.
- 37, , , et al. Second-generation line probe assay for hepatitis C virus genotyping. J Clin Microbiol 1996; 34: 2259–66.
- 38, , , et al. Hepatitis C virus genotyping by means of 5′-UR/core line probe assays and molecular analysis of untypeable samples. Virus Res 1995; 38: 137–57.
- 39, , , et al. Direct comparison of hepatitis C virus genotypes tested by INNO-LiPA HCV II and TRUGENE HCV genotyping methods. J Clin Virol 2003; 28: 214–6.
- 40, , Review article: specifically targeted anti-viral therapy for hepatitis C − a new era in therapy. Aliment Pharmacol Ther 2010; 32: 14–28.
- 41, , , et al. Hepatitis C virus (HCV) genotype 1 subtype identification in new HCV drug development and future clinical practice. PLoS One 2009; 4: e8209.
- 42
- 43, , , et al. A long-term study of hepatitis C virus replication in non-A, non-B hepatitis. N Engl J Med 1991; 325: 98–104.
- 44, , , et al. IgM antibody to hepatitis C virus in acute and chronic hepatitis C. Hepatology 1991; 14: 38–43.
- 45, , , et al. Twelve weeks post-treatment follow-up is as relevant as 24 weeks to determine the SVR in patients with HCV receiving PEG-IFN and ribavirin. Hepatology 2010; 51: 1122–6.
- 46, , , et al. Prophylaxis, diagnosis and therapy of hepatitis c virus (HCV) infection: the German guidelines on the management of HCV infection. Z Gastroenterol 2010; 48: 289–351.
- 47, , , et al. Peginterferon alpha-2a plus ribavirin for chronic hepatitis C virus infection. N Engl J Med 2002; 347: 975–82.
- 48, , , et al. Early virologic response to treatment with peginterferon alpha-2b plus ribavirin in patients with chronic hepatitis C. Hepatology 2003; 38: 645–52.
- 49, , , et al. Predicting sustained virological responses in chronic hepatitis C patients treated with peginterferon alpha-2a (40 KD)/ribavirin. J Hepatol 2005; 43: 425–33.
- 50, , , et al. Extended treatment duration for hepatitis C virus type 1: comparing 48 versus 72 weeks of peginterferon-alpha-2a plus ribavirin. Gastroenterology 2006; 130: 1086–97.
- 51, , , et al. Prediction of treatment outcome in patients with chronic hepatitis C: significance of baseline parameters and viral dynamics during therapy. Hepatology 2003; 37: 600–9.
- 52, , TeraViC-4 Study Group et al. Peginterferon-alfa2a plus ribavirin for 48 versus 72 weeks in patients with detectable hepatitis C virus RNA at week 4 of treatment. Gastroenterology 2006; 131: 451–60.
- 53, , , et al. COMPLETELY Individualized treatment durations (24, 30, 36, 42, 48, 60 OR 72 Weeks) with peginterferon-alfa-2B and ribavirin in hcv genotype 1-infected patients (INDIV-2 STUDY). J Hepatol 2010; 52 (Suppl. 1): S25–6.
- 54, , , et al. Colchicine versus PEG-Interferon alpha 2b long term therapy: results of the 4 year copilot trial. J Hepatol 2008; 48(Suppl. 2): S4.
- 55, , , et al. HALT-C Trial Investigators. Prolonged therapy of advanced chronic hepatitis C with low dose peginterferon. N Engl J Med 2008; 359: 2429–41.
- 56, , , et al. Pegintron maintenance therapy in cirrhotic (Metavir F4) HCV patients, who failed to respond to interferon/ribavirin (IR) therapy: final results of the EPIC3 cirrhosis maintenance trial. J Hepatol 2009; 50: A49.
- 57, , , et al. Efficacy of 24 weeks treatment with peginterferon alpha-2b plus ribavirin in patients with chronic hepatitis C infected with genotype 1 and low pretreatment viremia. J Hepatol 2006; 44: 97–103.
- 58, , , et al. Early identification of HCV genotype 1 patients responding to 24 weeks peginterferon alpha-2a (40 kd)/ribavirin therapy. Hepatology 2006; 43: 954–60.
- 59, , , et al. Peginterferon alpha-2a and ribavirin for 24 weeks in hepatitis C type 1 and 4 patients with rapid virological response. Gastroenterology 2008; 135: 451–8.
- 60, , , et al. Peginterferon-alpha2a and ribavirin combination therapy in chronic hepatitis C: a randomized study of treatment duration and ribavirin dose. Ann Intern Med 2004; 140: 346–55.
- 61, , , et al. Insulin resistance impairs rapid virologic response in HIV/hepatitis C virus coinfected patients on peginterferon-alpha-2a. AIDS 2008; 22: 857–61.
- 62, , Treatment predictors of a sustained virologic response in hepatitis B and C. J Hepatol 2008; 49: 634–51.
- 63, , Treatment extension to 72 weeks of peginterferon and ribavirin in hepatitis c genotype 1-infected slow responders. Hepatology 2007; 46: 1688–94.
- 64, , , et al. Individualized treatment duration for hepatitis C genotype 1 patients: A randomized controlled trial. Hepatology 2008; 47: 43–50.
- 65, , , et al. Customizing treatment with peginterferon alpha-2a (40KD) plus ribavirin in patients with HCV genotype 1 or 4 infection: interimresults of a prospective randomized trial. Hepatology 2006; 44: 336A.
- 66, , SUCCESS Study Investigators et al. Randomized trial of peginterferon alfa-2b and ribavirin for 48 or 72 weeks in patients with hepatitis C virus genotype 1 and slow virologic response. Hepatology 2010; 52: 1201–7.
- 67, , , et al. Peginterferon alpha-2a and ribavirin for 16 or 24 weeks in HCV genotype 2 or 3. N Engl J Med 2007; 357: 124–3.
- 68, , , et al. Treatment with pegylated interferon and ribavarin in HCV infection with genotype 2 or 3 for 14weeks: a pilot study. Hepatology 2004; 40: 1260–5.
- 69, , , et al. Peginterferon-alpha-2a (40KD) and ribavirin for 16 or 24 weeks in patients with genotype 2 or 3 chronic hepatitis C. Gastroenterology 2005; 129: 522–7.
- 70, , , et al. Peginterferon alpha-2b and ribavirin for 12 vs. 24 weeks in HCV genotype 2 or 3. N Engl J Med 2005; 352: 2609–17.
- 71, , , et al. A randomised study of peginterferon and ribavirin for 16 versus 24 weeks in patients with genotype 2 chronic hepatitis C. Gut 2007; 56: 553–9.
- 72, , , et al. Pegylated interferon alpha and ribavirin for 14 versus 24 weeks in patients with hepatitis C virus genotype 2 or 3 and rapid virological response. Hepatology 2008; 47: 35–42.
- 73, , , et al. Identifying hepatitis C virus genotype 2/3 patients who can receive a 16-week abbreviated course of peginterferon alfa-2a (40KD) plus ribavirin. Hepatology 2010; 51: 1897–903.
- 74, , , et al. Short-term treatment duration for HCV-2 and HCV-3 infected patients. Dig Liver Dis 2006; 38: 741–8.
- 75, , , et al. A randomized controlled trial of pegylated interferon alpha-2a (40 KD) or interferon alpha-2a plus ribavirin and amantadine v. interferon alpha-2a and ribavirin in treatment-naive patients with chronic hepatitis C. J Viral Hepat 2005; 12: 292–9.
- 76, , , et al. Should treatment with peginterferon plus ribavirin be intesified in patients with genotype 2/3 without a rapid virological response? # 8. J Hepatol 2007; 46(Suppl. 1): S6.
- 77, , , et al. Definition of rapid virologic response with a highly sensitive real-time PCR-based HCV RNA assay in peginterferon alfa-2a plus ribavirin response-guided therapy. J Hepatol 2010; 52: 832–8.
- 78, , , et al. Positive and negative prediction of sustained virologic response at weeks 2 and 4 of treatment with albinterferon alfa-2b or peginterferon alfa-2a in treatment-naïve patients with genotype 1, chronic hepatitis C. J Hepatol 2009; 51: 21–8.
- 79, , , et al. Virological response at 4 weeks to predict outcome of hepatitis C treatment with pegylated interferon and ribavirin. Antivir Ther 2009; 14: 501–11.
- 80, , , Review article: predicting response in hepatitis C virus therapy. Aliment Pharmacol Ther 2006; 23: 1043–54.
- 81, , , et al. Genetic variation in IL28B predicts hepatitis C treatment-induced viral clearance. Nature 2009; 461: 399–401.
- 82, , , et al. Interleukin-28B Polymorphism Improves Viral Kinetics and Is the Strongest Pretreatment Predictor of Sustained Virologic Response in Genotype 1 Hepatitis C Virus. Gastroenterology 2010; 139: 120–9.
- 83, , , et al. IL28B is associated with response to chronic hepatitis C interferonalpha and ribavirin therapy. Nat Genet 2009; 41: 1100–4.
- 84, , , et al. Genome-wide association of IL28B with response to pegylated interferonalpha and ribavirin therapy for chronic hepatitis C. Nat Genet 2009; 41: 1105–9.
- 85Genetic polymorphism and response to treatment in chronic hepatitis C: the future of personalized medicine. J Hepatol 2010; 52: 452–4.
- 86, , , et al. IFN-lambdas mediate antiviral protection through a distinct class II cytokine receptor complex. Nat Immunol 2003; 4: 69–77.
- 87, , , et al. An IL28B polymorphism determines treatment response of hepatitis C virus genotype 2 or 3 patients who do not achieve a rapid virologic response. Gastroenterology 2010; 139: 821–7.

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