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liv2724-sup-0001-figureS1.docxWord document450KFig. S1. Hepatic canalicular transporters, multidrug resistance-associated protein (Mrp) 3, 4-6, and multi-drug resistance protein 2 (Mdr2), mRNA expression. mRNA was quantified using branched DNA signal amplification assay. Data presented as average relative light unit (RLU) per 10μg total RNA ± SEM (n = 4–5 mice per group). * represents statistically significant difference between sham and bile-duct ligated (BDL) mice of same treatment group (P ≤ 0.05). represents statistically significant difference between JFE and vehicle treatment of same surgical group mice (P ≤ 0.05).
liv2724-sup-0002-figureS2.docxWord document89KFig. S2. Protein quantification of Ntcp and Bsep western blot results. Data are presented as mean relative protein expression (n = 2 per group).
liv2724-sup-0003-figureS3.docxWord document116KFig. S3. Myeloperoxidase (MPO) levels were quantified in liver. Data are presented as average MPO levels (U/L) ± SEM (n = 4–5 mice per group). MPO activity in liver was determined using an MPO assay kit (Cell technology, Mountain View, CA). * represents statistically significant difference between sham and bile-duct ligated (BDL) mice of same treatment group (P ≤ 0.05).
liv2724-sup-0004-figureS4.docxWord document30185KFig. S4. Immunohistochemical staining of liver sections for macrophages. (A) Immunohistochemical staining of sham mice livers for F4/80 antibody. (B) Immunohistochemical staining of sham mice livers for CD68 antibody.
liv2724-sup-0005-tableS1.docxWord document64KTable S1. Primer sequences.

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