The grasshopper Podisma tyatiensis, which is distributed only at the summit of Mount Tyatya on Kunashiri Island, the Kuril Islands, is closely related to Podisma sapporensis, which has a broad distribution range on the islands of northern Japan and the Russian Far East (Hokkaido, Sakhalin and Kunashiri). The present study examined the taxonomic status of P. tyatiensis by crossing P. tyatiensis males with P. sapporensis females from Sakhalin. More than 90% of eggs from intrapopulation crosses developed to at least the last embryonic stage, whereas only 64% of eggs from the interpopulation crosses developed into that embryonic stage. Cytogenetic observations of prediapause embryos showed that the interpopulation crosses always led to the production of unfertilized eggs, and that all of the developing embryos had the maternal genome only. A mixture of haploid and diploid cells of maternal origin was found in most of those embryos. This result shows that unfertilized eggs produced by P. sapporensis females from Sakhalin developed parthenogenetically to at least the embryonic stage before hatching. The present crossing experiments revealed a high level of incompatibility between the genomes of the Sakhalin population and the Tyatya population, and confirmed the full species status of P. tyatiensis.