Effects of saline intake on spermatophore and sperm ejaculation in the male swallowtail butterfly Papilio xuthus (Lepidoptera: Papilionidae)
Article first published online: 24 JUN 2005
Volume 8, Issue 2, pages 161–166, June 2005
How to Cite
WATANABE, M. and KAMIKUBO, M. (2005), Effects of saline intake on spermatophore and sperm ejaculation in the male swallowtail butterfly Papilio xuthus (Lepidoptera: Papilionidae). Entomological Science, 8: 161–166. doi: 10.1111/j.1479-8298.2005.00114.x
- Issue published online: 24 JUN 2005
- Article first published online: 24 JUN 2005
- Received 28 May 2004; accepted 31 January 2005.
- apyrene spermatozoon;
- eupyrene sperm bundle;
- saline concentration;
- spermatophore size
Although sodium ions induce puddling behavior in males of some butterfly species, the role of sodium ions in male life history is unclear. Newly emerged laboratory-reared males of the swallowtail butterfly Papilio xuthus were allocated to five adult diets: water (as a control) and 0.001, 0.01, 0.1 or 1 mol/L saline solution. Males preferred to take 0.1 mol/L saline solution. We attempted to hand-pair males after two days of intake. Males on all diets, except those taking 1 mol/L saline solution, were likely to couple. Males taking 0.01 mol/L saline solution produced the largest spermatophore, although there were no significant differences in the number of eupyrene sperm bundles and apyrene spermatozoa between saline-taking males and control males. Salt accumulation in the male body also had little effect on the number of both types of spermatozoon in the spermatophore.