Promotion of cutaneous wound healing by local application of mesenchymal stem cells derived from human umbilical cord blood

Authors

  • Gaoxing Luo MD, PhD,

    1. State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing, China,
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    • *Contributed equally to this work.

  • Wenguang Cheng MD,

    1. State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing, China,
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    • *Contributed equally to this work.

  • Weifeng He PhD,

    1. State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing, China,
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  • Xiaojuan Wang MD, PhD,

    1. State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing, China,
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  • Jiangling Tan MD,

    1. State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing, China,
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  • Mark Fitzgerald MD,

    1. Trauma service, Alfred Hospital, Melbourne, Vic., Australia, and
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  • Xianchang Li PhD,

    1. Harvard Medical School, Transplant Research Center, Beth Israel Deaconess Medical Center, Boston, Massachusetts
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  • Jun Wu MD

    1. State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing, China,
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Reprint requests:
Gaoxing Luo, MD, PhD, State Key Laboratory of Trauma, Burn and Combined Injury, Burn Research Institute, Southwestern Hospital, Third Military Medical University, Chongqing 400038, China.
Tel:+86 23 68752688;
Fax:+86 23 68752688;
E-mail:logxw@hotmail.com

ABSTRACT

This study aimed to determine whether mesenchymal stem cells (MSCs) derived from umbilical cord blood (UCB) would promote cutaneous wound healing. MSCs from human UCB were isolated and identified. The characteristics of the isolated MSCs' growth and proliferation were assayed in vitro. The MSCs labeled with 5-bromodeoxyuridine (BrdU) were applied on fresh cutaneous mice wounds. The healing rates were surveyed. The distribution and the differentiation into keratinocytes of the labeled MSCs in the wound tissue were checked by immunohistochemistry staining. The isolated MSCs could grow and proliferate well in vitro. The isolated MSCs from UCB could be labeled by 5-bromodeoxyuridine successfully. The MSCs derived from UCB could enhance the healing of mice skin defect wounds, and it was found that the implanted MSCs could differentiate into keratinocyte in the wound tissue. It was demonstrated that MSCs from UCB can be isolated and proliferated successfully. The local administration of MSCs derived from UCB improves skin defect wound healing in mice.

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