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Fig. S1. DAC and BAB2 in species other than D. melanogaster Everting pupal leg disks were stained with anti-DAC or anti-BAB2. Antibody is indicated upper left. Black arrowheads indicate prothoracic ts2 primordia. (A) DAC in prothoracic leg disc from D. takahashi (two combs). Staining is weaker in future ts2 than in ts1 cells. (B) Male and female D. kikkawai and D. pseudoobscura larvae were selected at L3 and allowed to form pupae. Pupal disks were stained with anti-BAB2. Sex and species are indicated below. Female and male D. kikkawai or D. pseudoobscura prothoracic disks show no detectable BAB2 differences. (C) BAB2 in pupal disc from D. virilis forms a PD gradient similar to that of Sophophora species (compare to Figs. 3B, 6C, and above).

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EDE_219_sm_fig_S1.tif350KSupporting info item
EDE_fig_s1.tif350KSupporting info item

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