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Using the same poly(A)+RNA preparation used for performing RT-PCR reaction in the main text, 1st strand cDNA synthesis was done for each Oh and 14 h AEL RNA samples using PrimeScript™ 1st strand cDNA synthesis kit (Takara) with or without reverse transcriptase. PCR reactions were done using these cDNA as described in the Materials and Methods section in the main text except for the PCR cycles (PCR cycles for nanosM,N and O was 30 and that for nanosP was 35). Specific band was detected only when reverse transcriptase was included in the 1st strand cDNA synthesis reactions (below).

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