1Present address: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724-2209, USA
Sequence and function of basic helix–loop–helix proteins required for stomatal development in Arabidopsis are deeply conserved in land plants
Article first published online: 16 MAR 2011
© 2011 Wiley Periodicals, Inc.
Evolution & Development
Volume 13, Issue 2, pages 182–192, March/April 2011
How to Cite
MacAlister, C. A. and Bergmann, D. C. (2011), Sequence and function of basic helix–loop–helix proteins required for stomatal development in Arabidopsis are deeply conserved in land plants. Evolution & Development, 13: 182–192. doi: 10.1111/j.1525-142X.2011.00468.x
- Issue published online: 16 MAR 2011
- Article first published online: 16 MAR 2011
SUMMARY Stomata are a broadly conserved feature of land plants with a crucial role regulating transpiration and gas exchange between the plant and atmosphere. Stereotyped cell divisions within a specialized cell lineage of the epidermis generate stomata and define the pattern of their distribution. The behavior of the stomatal lineage varies in its detail among different plant groups, but general features include asymmetric cell divisions and an immediate precursor (the guard mother cell [GMC]) that divides symmetrically to form the pair of cells that will differentiate into the guard cells. In Arabidopsis, the closely related basic helix–loop–helix (bHLH) subgroup Ia transcription factors SPEECHLESS, MUTE, and FAMA promote asymmetric divisions, the acquisition of GMC identity and guard cell differentiation, respectively. Genome sequence data indicate that these key positive regulators of stomatal development are broadly conserved among land plants. While orthologies can be established among individual family members within the angiosperms, more distantly related groups contain subgroup Ia bHLHs of unclear affinity. We demonstrate group Ia members from the moss Physcomitrella patens can partially complement MUTE and FAMA and recapitulate gain of function phenotypes of group Ia genes in multiple steps in the stomatal lineage in Arabidopsis. Our data are consistent with a mechanism whereby a multifunctional transcription factor underwent duplication followed by specialization to provide the three (now nonoverlapping) functions of the angiosperm stomatal bHLHs.