W.Y. Kim and J.-W. Lee contributed equally to this paper.
Increased expression of Toll-like receptor 5 during progression of cervical neoplasia
Article first published online: 22 JUN 2007
© 2007, Copyright the Authors
International Journal of Gynecological Cancer
Volume 18, Issue 2, pages 300–305, March/April 2008
How to Cite
KIM, W.Y., LEE, J.-W., CHOI, J.-J., CHOI, C.H., KIM, T.-J., KIM, B.-G., SONG, S.Y. and BAE, D.-S. (2008), Increased expression of Toll-like receptor 5 during progression of cervical neoplasia. International Journal of Gynecological Cancer, 18: 300–305. doi: 10.1111/j.1525-1438.2007.01008.x
- Issue published online: 20 JUL 2007
- Article first published online: 22 JUN 2007
- Accepted for publication April 24, 2007
- cervical neoplasia;
- squamous epithelium;
- Toll-like receptor 5
The purpose of this study was to determine whether Toll-like receptor 5 (TLR5) expression was associated with disease progression in cervical neoplasia. TLR5 expression was evaluated by immunohistochemistry (IHC) in 55 formalin-fixed paraffin-embedded cervical tissues; 10 normal cervical specimens, 9 low-grade cervical intraepithelial neoplasias (CINs), 12 high-grade CINs, and 24 invasive squamous cell carcinomas (ISCCs). TLR5 expression was also evaluated at the RNA level, in fresh, frozen cervical carcinoma tissues by real-time quantitative RT-PCR. TLR5 expression, which was mainly observed as cytoplasmic staining, gradually increased in accordance with the histopathologic grade in the following order: low-grade CIN less than high-grade CIN less than ISCC (P < 0.001). Immunohistochemical staining showed that TLR5 expression was undetectable (80%) or weak (20%) in normal cervical squamous epithelial tissues. However, moderate expression was detected in 33.3% of low-grade CIN (3/9), 41.7% of high-grade CIN (5/12), and 45.8% of ISCC (11/24). Strong expression was detected in as much as 33.3% of high-grade CIN (4/12) and 50% of ISCC (12/24). Contrary to IHC results, real-time quantitative RT-PCR revealed that TLR5 expression in tumors was not statistically different compared to normal cervical tissues (P = 0.1452). The IHC result suggests that TLR5 may play a significant role in tumor progression of cervical neoplasia and may represent a useful marker for malignant transformation of cervical squamous cells.