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High-intensity electric pulses induce mitochondria-dependent apoptosis in ovarian cancer xenograft mice

Authors


Li Na Hu, MD, Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Sichuan University, 37 Guoxue Road, Chengdu 610081, China. Email: lcxmm@yahoo.com.cn

Abstract

Human ovarian cancer models were established in nude mice by transplanting SKOV3 cells, and then tumors were exposed to high-intensity electric pulses with a voltage 1000 V, frequency of 1000 Hz, and duration of 250 ns for 1 min. Mitochondria permeability transition pore (PTP) was inspected by cofocal microscope; cytochrome C (Cyt C) and apoptosis-induced factor (AIF) were determined by immunohistochemistry; and voltage-dependent anion channel (VDAC) was measured by immunofluorescence. High-intensity electric pulses exposure led to increases of PTP, Cyt C, and AIF and a decrease of VDAC. These findings revealed that high-intensity electric pulses activated mitochondria electroporation, apoptosis was realized via mitochondria pathway.

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