The first two authors contributed equally to this work.
Age Dependence of the Human Skeletal Muscle Stem Cell in Forming Muscle Tissue
Article first published online: 16 FEB 2006
Volume 30, Issue 3, pages 130–140, March 2006
How to Cite
Schäfer, R., Knauf, U., Zweyer, M., Högemeier, O., De Guarrini, F., Liu, X., Eichhorn, H. J., Koch, F. W., Mundegar, R. R., Erzen, I. and Wernig, A. (2006), Age Dependence of the Human Skeletal Muscle Stem Cell in Forming Muscle Tissue. Artificial Organs, 30: 130–140. doi: 10.1111/j.1525-1594.2006.00199.x
- Issue published online: 16 FEB 2006
- Article first published online: 16 FEB 2006
- Received June 2005; revised September 2005.
- Human myoblast;
- Muscle stem cell;
Abstract: Human skeletal muscle stem cells from healthy donors aged 2–82 years (n = 13) and from three children suffering from Duchenne Muscular Dystrophy (DMD) were implanted into soleus muscles of immunoincompetent mice and were also expanded in vitro until senescence. Growth of implanted cells was quantified by structural features and by the amount of human DNA present in a muscle. Proliferative capacity in vitro and in vivo was inversely related to age of the donor. In vitro, a decline of about two mean population doublings (MPDs) per 10 years of donor’s age was observed. Muscle stem cells from DMD children were prematurely aged. In general, cell preparations with low or decreasing content in desmin-positive cells produced more MPDs than age-matched high-desmin preparations and upon implantation more human DNA and more nonmyogenic than myogenic tissue. Thus, a “Desmin Factor” was derived which predicts “quality” of the human muscle tissue growing in vivo. This factor may serve as a prognostic tool.