Abdominal Hernia Repair With a Decellularized Dermal Scaffold Seeded With Autologous Bone Marrow-Derived Mesenchymal Stem Cells
Article first published online: 7 SEP 2011
© 2011, Copyright the Authors. Artificial Organs © 2011, International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.
Volume 36, Issue 3, pages 247–255, March 2012
How to Cite
Zhao, Y., Zhang, Z., Wang, J., Yin, P., Zhou, J., Zhen, M., Cui, W., Xu, G., Yang, D. and Liu, Z. (2012), Abdominal Hernia Repair With a Decellularized Dermal Scaffold Seeded With Autologous Bone Marrow-Derived Mesenchymal Stem Cells. Artificial Organs, 36: 247–255. doi: 10.1111/j.1525-1594.2011.01343.x
- Issue published online: 6 MAR 2012
- Article first published online: 7 SEP 2011
- Received December 2010; revised April 2011.
- Bone–marrow-derived mesenchymal stem cells;
- Abdominal wall hernia;
Surgeons usually use synthetic polymer meshes for abdominal wall hernia repair. However, synthetic polymer meshes exhibit a lack of growth and related complications. In this study, we produced a tissue-engineered patch for abdominal hernia repair. Autologous bone–marrow-derived mesenchymal stem cells (BMSCs) were isolated and proliferated in vitro; decellularized dermal scaffolds (DSs) were prepared using enzymatic process; and then BMSCs were seeded onto the DSs for the construction of tissue-engineered patches. Under general anesthesia, rabbits underwent creation of abdominal wall defects and which were repaired with BMSC-seeded DSs, acellular DSs, and skin sutures only, respectively. Animals were sacrificed after 2 months for assessing the histological and gross examination. Abdominal hernias were absent in animals repaired with cell-seeded group, and abdominal hernias or bulges appeared in all animals repaired with acellular group. All the animals that were not repaired died within 10 days. The cell-seeded implants were thicker and indicated good angiogenesis compared with that of the acellular implants, both in histological and gross examination. The tissue-engineered patches prepared with BMSCs seeding on DSs can be used for abdominal wall hernia repair.