5-HT 2 Serotonin Receptor on Blood Platelet of Migraine Patients

Authors

  • Piyarat Govitrapong Ph.D.,

    Corresponding author
    1. Neuro and Behavioral Biology Center, Institute of Science and Technology for Development, Mahidol University at Salaya, Nakornpathom 73170, Thailand and
      Piyarat Govitrapong, Ph.D. Department of Pharmacology, University of Minnesota Medical Center, 3-249 Millard Hall, 435 Delaware Street SE Minneapolis, MN 55455.
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  • Chanchira Limthavon M.S.,

    1. Neuro and Behavioral Biology Center, Institute of Science and Technology for Development, Mahidol University at Salaya, Nakornpathom 73170, Thailand and
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  • Anan Srikiatkhachorn M.D.

    1. Neuro and Behavioral Biology Center, Institute of Science and Technology for Development, Mahidol University at Salaya, Nakornpathom 73170, Thailand and
    2. Neurology Clinic Unit, Chulalongkorn University Hospital, Bangkok, Thailand.
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Piyarat Govitrapong, Ph.D. Department of Pharmacology, University of Minnesota Medical Center, 3-249 Millard Hall, 435 Delaware Street SE Minneapolis, MN 55455.

Abstract

SYNOPSIS

Several lines of evidence have previously suggested that platelets might play a primary or secondary role in migraine pathogenesis, and that in addition serotonin receptors on human platelets might be involved in those processes. By using [3H]-spiperone as a radioligand and ketanserin to determine the non-specific binding, the numbers of 5-HT2 serotonin receptors on platelet membrane obtained from normal healthy and migraine subjects were determined. We found a significant decrease (p <0.02) in the maximal receptor numbers (Bmax) on platelet membrane of migraine patients (Bmax= 33.01±5.57 fmol/mg protein ) when compared to the normal healthy group (Bmax=86.59±9.09 fmol/mg protein) whereas the dissociation equilibrium constant (Kd) values (2.47±0.44 nM and 3.41±0.95 nM for the normal and migraine subjects, respectively) remained unchanged. The platelet response showed a higher degree of agreeability in migraine subjects, whereas the platelet counts were the same. This finding implies that serotonin receptors on the platelet may reflect some aspect of the pathogenesis of migraine headache.

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