Lack of Association of Endothelial Nitric Oxide Synthase Polymorphisms and Migraine

Authors

  • María Toriello,

    1. From the Services of Neurology, University Hospital “Marqués de Valdecilla,” Santander, Spain (M. Toriello, A. Oterino, and A. Alonso-Arranz); Service of Neurology, University Hospital of Salamanca, Salamanca, Spain (J. Pascual); Health Center of Camargo, Cantabria, Spain (J. Castillo); Health Center of Santoña, Cantabria, Spain (R. Colás); Microbiology, University Hospital “Marqués de Valdecilla,” Santander, Spain (C. Ruiz-Alegría); Health Center of Polanco, Cantabria, Spain (E. Quintela); Neurology Service of the “La Candelaria” Hospital, Tenerife, Spain (F. Montón and N. Ruiz-Lavilla).
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  • Agustín Oterino MD,

    1. From the Services of Neurology, University Hospital “Marqués de Valdecilla,” Santander, Spain (M. Toriello, A. Oterino, and A. Alonso-Arranz); Service of Neurology, University Hospital of Salamanca, Salamanca, Spain (J. Pascual); Health Center of Camargo, Cantabria, Spain (J. Castillo); Health Center of Santoña, Cantabria, Spain (R. Colás); Microbiology, University Hospital “Marqués de Valdecilla,” Santander, Spain (C. Ruiz-Alegría); Health Center of Polanco, Cantabria, Spain (E. Quintela); Neurology Service of the “La Candelaria” Hospital, Tenerife, Spain (F. Montón and N. Ruiz-Lavilla).
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  • Julio Pascual MD,

    1. From the Services of Neurology, University Hospital “Marqués de Valdecilla,” Santander, Spain (M. Toriello, A. Oterino, and A. Alonso-Arranz); Service of Neurology, University Hospital of Salamanca, Salamanca, Spain (J. Pascual); Health Center of Camargo, Cantabria, Spain (J. Castillo); Health Center of Santoña, Cantabria, Spain (R. Colás); Microbiology, University Hospital “Marqués de Valdecilla,” Santander, Spain (C. Ruiz-Alegría); Health Center of Polanco, Cantabria, Spain (E. Quintela); Neurology Service of the “La Candelaria” Hospital, Tenerife, Spain (F. Montón and N. Ruiz-Lavilla).
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  • Jesús Castillo MD,

    1. From the Services of Neurology, University Hospital “Marqués de Valdecilla,” Santander, Spain (M. Toriello, A. Oterino, and A. Alonso-Arranz); Service of Neurology, University Hospital of Salamanca, Salamanca, Spain (J. Pascual); Health Center of Camargo, Cantabria, Spain (J. Castillo); Health Center of Santoña, Cantabria, Spain (R. Colás); Microbiology, University Hospital “Marqués de Valdecilla,” Santander, Spain (C. Ruiz-Alegría); Health Center of Polanco, Cantabria, Spain (E. Quintela); Neurology Service of the “La Candelaria” Hospital, Tenerife, Spain (F. Montón and N. Ruiz-Lavilla).
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  • Rafael Colás MD,

    1. From the Services of Neurology, University Hospital “Marqués de Valdecilla,” Santander, Spain (M. Toriello, A. Oterino, and A. Alonso-Arranz); Service of Neurology, University Hospital of Salamanca, Salamanca, Spain (J. Pascual); Health Center of Camargo, Cantabria, Spain (J. Castillo); Health Center of Santoña, Cantabria, Spain (R. Colás); Microbiology, University Hospital “Marqués de Valdecilla,” Santander, Spain (C. Ruiz-Alegría); Health Center of Polanco, Cantabria, Spain (E. Quintela); Neurology Service of the “La Candelaria” Hospital, Tenerife, Spain (F. Montón and N. Ruiz-Lavilla).
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  • Ana Alonso-Arranz,

    1. From the Services of Neurology, University Hospital “Marqués de Valdecilla,” Santander, Spain (M. Toriello, A. Oterino, and A. Alonso-Arranz); Service of Neurology, University Hospital of Salamanca, Salamanca, Spain (J. Pascual); Health Center of Camargo, Cantabria, Spain (J. Castillo); Health Center of Santoña, Cantabria, Spain (R. Colás); Microbiology, University Hospital “Marqués de Valdecilla,” Santander, Spain (C. Ruiz-Alegría); Health Center of Polanco, Cantabria, Spain (E. Quintela); Neurology Service of the “La Candelaria” Hospital, Tenerife, Spain (F. Montón and N. Ruiz-Lavilla).
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  • Carlos Ruiz-Alegría MD,

    1. From the Services of Neurology, University Hospital “Marqués de Valdecilla,” Santander, Spain (M. Toriello, A. Oterino, and A. Alonso-Arranz); Service of Neurology, University Hospital of Salamanca, Salamanca, Spain (J. Pascual); Health Center of Camargo, Cantabria, Spain (J. Castillo); Health Center of Santoña, Cantabria, Spain (R. Colás); Microbiology, University Hospital “Marqués de Valdecilla,” Santander, Spain (C. Ruiz-Alegría); Health Center of Polanco, Cantabria, Spain (E. Quintela); Neurology Service of the “La Candelaria” Hospital, Tenerife, Spain (F. Montón and N. Ruiz-Lavilla).
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  • Estrella Quintela MD,

    1. From the Services of Neurology, University Hospital “Marqués de Valdecilla,” Santander, Spain (M. Toriello, A. Oterino, and A. Alonso-Arranz); Service of Neurology, University Hospital of Salamanca, Salamanca, Spain (J. Pascual); Health Center of Camargo, Cantabria, Spain (J. Castillo); Health Center of Santoña, Cantabria, Spain (R. Colás); Microbiology, University Hospital “Marqués de Valdecilla,” Santander, Spain (C. Ruiz-Alegría); Health Center of Polanco, Cantabria, Spain (E. Quintela); Neurology Service of the “La Candelaria” Hospital, Tenerife, Spain (F. Montón and N. Ruiz-Lavilla).
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  • Fernando Montón MD,

    1. From the Services of Neurology, University Hospital “Marqués de Valdecilla,” Santander, Spain (M. Toriello, A. Oterino, and A. Alonso-Arranz); Service of Neurology, University Hospital of Salamanca, Salamanca, Spain (J. Pascual); Health Center of Camargo, Cantabria, Spain (J. Castillo); Health Center of Santoña, Cantabria, Spain (R. Colás); Microbiology, University Hospital “Marqués de Valdecilla,” Santander, Spain (C. Ruiz-Alegría); Health Center of Polanco, Cantabria, Spain (E. Quintela); Neurology Service of the “La Candelaria” Hospital, Tenerife, Spain (F. Montón and N. Ruiz-Lavilla).
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  • Nuria Ruiz-Lavilla MD

    1. From the Services of Neurology, University Hospital “Marqués de Valdecilla,” Santander, Spain (M. Toriello, A. Oterino, and A. Alonso-Arranz); Service of Neurology, University Hospital of Salamanca, Salamanca, Spain (J. Pascual); Health Center of Camargo, Cantabria, Spain (J. Castillo); Health Center of Santoña, Cantabria, Spain (R. Colás); Microbiology, University Hospital “Marqués de Valdecilla,” Santander, Spain (C. Ruiz-Alegría); Health Center of Polanco, Cantabria, Spain (E. Quintela); Neurology Service of the “La Candelaria” Hospital, Tenerife, Spain (F. Montón and N. Ruiz-Lavilla).
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  • Conflict of Interest: None

A. Oterino Durán, Service of Neurology, University Hospital “Marqués de Valdecilla,” Santander—E 39008, Spain.

Abstract

Objective.— The aim of this study was to evaluate if 2 functional endothelial nitric oxide synthase (eNOS) gene polymorphisms might be risk factors for migraine.

Background.— Nitric oxide synthase promotes the synthesis of nitric oxide (NO). NO is a potent vasodilator and mediates several processes involved in migraine pathophysiology. Only one study has suggested an association with migraine with aura.

Methods.— We performed a sex- and age-matched case-control study using 2 eNOS polymorphisms (rs1800779 and rs1799983), which are in linkage disequilibrium. Genotypes were obtained with allele-specific probes in a real-time polymerase chain reaction assay. Genotypic and allelic distributions were compared with χ2 method. We also estimated the reconstructed haplotypes and calculated ORs for individual haplotypes.

Results.— A total of 337 migraine patients (188 with aura) and 341 healthy controls were recruited. We found no significant differences in the distribution of genotypes and alleles for either polymorphism among clinical subgroups. Neither rs1800779 nor rs1799983 polymorphisms increased the risk for suffering from migraine aura.

Conclusions.— As others have previously reported, we failed to demonstrate genetic association of the eNOS gene with migraine.

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