Accepted: 21 October 1981.
PHYSIOLOGICAL VARIATION AND ELECTROPHORETIC BANDING PATTERNS OF GENETICALLY DIFFERENT SEASONAL POPULATIONS OF SKELETONEMA COSTATUM (BACILLARIOPHYCEAE)1
Article first published online: 25 NOV 2004
Journal of Phycology
Volume 18, Issue 1, pages 148–162, MARCH 1982
How to Cite
Gallagher, J. C. (1982), PHYSIOLOGICAL VARIATION AND ELECTROPHORETIC BANDING PATTERNS OF GENETICALLY DIFFERENT SEASONAL POPULATIONS OF SKELETONEMA COSTATUM (BACILLARIOPHYCEAE). Journal of Phycology, 18: 148–162. doi: 10.1111/j.1529-8817.1982.tb03169.x
Ken Brooks, Alvan Colon, John Kempisty, and Elena Seon helped with the cell counts and chemical analyses. My thanks go to Robert R. L. Guillard for providing inocula of clones Skel and epSkel, and to Robert L. Rockwell for critical evaluation of an earlier draft of the manuscript. This research was supported by grants 13058 and 13316 from the PSC-BHE research program of the Research Foundation of the City University of New York, and National Science Foundation Grant DEB-8021744.
- Issue published online: 25 NOV 2004
- Article first published online: 25 NOV 2004
- electrophoretic bands;
- genetic variation;
- Narragansett Bay;
- seasonal populations;
- Skeletonema costatum
Clones of Skeletonema costatum (Grev.) Cl. isolated from Narragansett Bay, R.I., during different seasons were grouped according to their electrophoretic banding patterns. The growth rates, pg chlorophyll · cell−1, carbon uptake · cell−1· h−1, and carbon uptake · pg chl−1· h−1 were measured at 20°C, in a 14:10 h L:D cycle at 180 μE · m−2· s−1. Statistically significant sources of variation were found among groups of clones in growth rate, pg chl · cell−1, and carbon uptake · pg chl−1· h−1. It was concluded that there is a significant relationship between the physiological characteristics of clones isolated from populations in different seasons and patterns of genetic variation inferred from the electrophoretic studies. However, genetic diversity detected by banding patterns tends to underestimate the total genetic diversity in natural populations. The groups of clones most common in summer bloom populations had significantly higher growth rates, lower values of pg chl · cell−1, and higher rates of carbon uptake · pg chl−1· h−1 at 20°C than did the group of clones most common in winter bloom populations. However, differences among groups in these parameters at 20°C alone cannot account for the seasonal cycling of genetically variable populations of Skeletonema in Narragansett Bay. The range of growth rates among clones of this species is 0.1–5.0 divisions · d−1 under a single set of temperature and light conditions. Chlorophyll concentrations range from 0.2–1.7 pg chl · cell−1 and carbon uptake · pg chl−1· h−1 varies by a factor of 7 among clones. The range of physiological variation in this species means that it is difficult to use laboratory studies of single clones to analyze the responses of natural populations of Skeletonema.