These authors contributed equally to this work.
Generation and analysis of 5318 expressed sequence tags from the filamentous sporophyte of Porphyra haitanensis (Rhodophyta)1
Article first published online: 7 DEC 2007
© 2007 Phycological Society of America
Journal of Phycology
Volume 43, Issue 6, pages 1287–1294, December 2007
How to Cite
Xiaolei, F., Yongjun, F., Songnian, H. and Guangce, W. (2007), Generation and analysis of 5318 expressed sequence tags from the filamentous sporophyte of Porphyra haitanensis (Rhodophyta). Journal of Phycology, 43: 1287–1294. doi: 10.1111/j.1529-8817.2007.00415.x
Received 14 June 2006. Accepted 9 April 2007.
- Issue published online: 7 DEC 2007
- Article first published online: 7 DEC 2007
- bioinformatic analyses;
- Porphyra haitanensis;
Porphyra haitanensis T. J. Chang et B. F. Zheng (Bangiales, Rhodophyta) is cultivated in China and widely consumed in Asia. To gain more insight into its physiological and biochemical properties, we generated 5318 expressed sequence tags (ESTs) from the sporophyte of P. haitanensis, and upon assembling into a nonredundant set, 2535 sequences were obtained, among which only 32.2% (816) shared certain similarity with published sequences (Nr and KOG). Functional classification of such ESTs revealed that most of the transcripts were related to its conservative biological metabolism, and P. haitanensis most likely possesses cyanide-resistant respiration and a C4-like carbon-fixation pathway, both of which have never been reported in a rhodophyte before. Twenty-eight percent of the nonredundant gene clusters exhibited significant similarity to those from P. yezoensis Ueda sporophytes, and 16 genes up-regulated in P. yezoensis sporophytes were also expressed abundantly in P. haitanensis. Codon usage analysis indicated that exposure to high GC pressure might occur during evolution of P. haitanensis. These findings represent the most extensive collection of ESTs from P. haitanensis to date, and all the ESTs in this study have been submitted to GenBank (accession nos. DN604790–DN608469, EG016226–EG018540).