INORGANIC CARBON ACQUISITION BY CHRYSOPHYTES1

Authors

  • Stephen C. Maberly,

    1. Centre for Ecology & Hydrology, Lancaster Environment Centre, Library Avenue, Bailrigg, Lancaster, LA1 4AP, UK
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  • Lucy A. Ball,

    1. The Freshwater Biological Association, The Ferry Landing, Far Sawrey, Ambleside, Cumbria, LA22 0LP, UK
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    • 3

      Present address: Centre for Ecology & Hydrology, Maclean Building, Crowmarsh Gifford, Wallingford, OX10 8BB, UK.

  • John A. Raven,

    1. Division of Plant Sciences, The University of Dundee at SCRI, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK
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  • Dieter Sültemeyer

    1. Fachbereich Biologie, Technische Universität Kaiserslautern, D-67663 Kaiserslautner, Germany
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  • 1

    Received 6 October 2008. Accepted 6 May 2009.

Abstract

Twelve species, representing 12 families of the chrysophytes sensu lato, were tested for their ability to take up inorganic carbon. Using the pH-drift technique, CO2 compensation points generally varied between 1 and 20 μmol · L−1 with a mean concentration of 5 μmol · L−1. Neither pH nor alkalinity affected the CO2 compensation point. The concentration of oxygen had a relatively minor effect on CO2-uptake kinetics, and the mean CO2 compensation point calculated from the kinetic curves was 3.6 μmol · L−1 at 10–15 kPa starting oxygen partial pressure and 3.8 μmol · L−1 at atmospheric starting oxygen partial pressure (21 kPa). Similarly, uptake kinetics were not affected by alkalinity, and hence concentration of bicarbonate. Membrane inlet mass spectrometry (MIMS) in the presence and absence of acetazolamide suggested that external carbonic anhydrase in Dinobryon sertularia Ehrenb. and Synura petersenii Korschikov was either very low or absent. Rates of net HCO3 uptake were very low (∼5% of oxygen evolution) using MIMS and decreased rather than increased with increasing HCO3 concentration, suggesting that it was not a real uptake. The CO2 compensation points determined by MIMS for CO2 uptake and oxygen evolution were similar to those determined in pH-drift and were >1 μmol · L−1. Overall, the results suggest that chrysophytes as a group lack a carbon-concentrating mechanism (CCM), or an ability to make use of bicarbonate as an alternative source of inorganic carbon. The possible evolutionary and ecological consequences of this are briefly discussed.

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