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DOES DINOPHYSIS CAUDATA (DINOPHYCEAE) HAVE PERMANENT PLASTIDS?1
Article first published online: 30 NOV 2009
© 2009 Phycological Society of America
Journal of Phycology
Volume 46, Issue 2, pages 236–242, April 2010
How to Cite
Park, M. G., Kim, M., Kim, S. and Yih, W. (2010), DOES DINOPHYSIS CAUDATA (DINOPHYCEAE) HAVE PERMANENT PLASTIDS?. Journal of Phycology, 46: 236–242. doi: 10.1111/j.1529-8817.2009.00777.x
Received 22 January 2009. Accepted 29 August 2009.
- Issue published online: 31 MAR 2010
- Article first published online: 30 NOV 2009
- permanent plastids;
- plastid preference;
- restriction fragment length polymorphism
The marine photosynthetic dinoflagellates Dinophysis Ehrenb. species are obligate mixotrophs that require both light and the ciliate prey Myrionecta rubra (= Mesodinium rubrum) for long-term survival. Despite rapid progress on the study of Dinophysis using laboratory cultures, however, whether it has its own permanent plastids or kleptoplastids (i.e., stolen plastids from its ciliate prey) is not fully resolved. Here, we addressed this issue using established cultures of D. caudata Saville-Kent strain DC-LOHABE01 and cross-feeding/starvation experiments encompassing the prey M. rubra strain MR-MAL01 cultures grown on two different cryptophytes (strains CR-MAL01 and CR-MAL11). To follow the fate of prey plastids, psbA gene as a tracer was amplified from individually isolated D. caudata cells, and the PCR products were digested with a restriction enzyme, SfaNI. The RFLP pattern of the PCR products digested by SfaNI revealed that D. caudata continued to keep CR-MAL01–type plastids, while it lost CR-MAL11–type plastids with increasing starvation time. Our results suggest that Dinophysis treats in different ways plastids taken up from different cryptophytes via its ciliate prey M. rubra. Alternatively, D. caudata may already have its own CR-MAL01–type permanent plastid, with two types of plastids (CR-MAL01 and CR-MAL11) obtained from M. rubra being lost within 1 month. This result highlights the need to identify more accurately the origin of plastids in newly isolated photosynthetic Dinophysis species to resolve the issue of plastid permanence.