SEARCH

SEARCH BY CITATION

Figure S1. Photomicrographs showing changes in the morphology and position of cells during the conjugation processes in a homothallic strain of the Closterium peracerosum–strigosum–littorale (C. psl.) complex (kodama20). Cells were photographed at (a) 0 h, (b) 6 h, (c) 12 h, (d) 18 h, (e) 24 h, (f) 30 h, (g) 36 h, (h) 42 h, and (i) 48 h after the onset of incubation in nitrogen-depleted mating (MI) medium. Scale bar = 500 μm.

Figure S2. Outcrossing zygospore formation in a homothallic strain of the Closterium peracerosum–strigosum–littorale (C. psl.) complex. Cells of kodama20 were cultured in nitrogen-depleted mating (MI) medium. Arrow indicates a clone of another individual. Scale bar = 50 μm. (a) Vegetative cell, (b) formation of septum, (c) cell division resulting in the formation of two sister gametangial cells from one vegetative cell, (d) formation of a sexual pair, (e) another clone approaching a sexual pair, (f) individual of a second clone approaching one of the sexual pair, (g) formation of conjugation papillae between a cell of the second clone and one of the sexual pair, and (h) formation of outcrossing zygospore by protoplast fusion.

Please note: Wiley-Blackwell are not responsible for the content or functionality of any supplementary materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.

FilenameFormatSizeDescription
JPY_806_sm_FigS1_a_c.tif4107KSupporting info item
JPY_806_sm_FigS1_d_f.tif4108KSupporting info item
JPY_806_sm_FigS1_g_i.tif4126KSupporting info item
JPY_806_sm_FigS2.tif11185KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.