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Figure S1. (A) Inferred phylogenetic relationship between complete eukaryotic assimilatory nitrate reductase amino acid sequences. (B) Inferred phylogenetic relationship between complete eukaryotic formamidase amino acid sequences. (C) Inferred phylogenetic relationship between complete plant, oomycetes, and algae glutamine synthetase II amino acid sequences.

Figure S2. (A) Alignment in ClustalW of the complete assimilatory nitrate reductase protein from Emiliania huxleyi (TPA BK005976), the pelagophyte Aureococcus anophagefferens (C_70101.Auran1), the diatom Phaeodactylum tricornutum (AAV66996.1), the chlorophyte Chlamydomonas reinhardtii (AF203033_1) and the vascular plant Arabidopsis thaliana (P11035). (B) Alignment in ClustalW of the amino acid sequences of the putative formamidase from E. huxleyi (TPA BK005975), the pelagophyte A. anophagefferens (C_140301|Auran1), the diatom P. tricornutum (Phatr1:21465), the chlorophyte C. reinhardtii (XP 001699344.1), the vascular plant A. thaliana (AAK59505.1), the cyanobacteria Synechococcus sp. WH 5701 (ZP 01084549) and the fungus A. fumigatus (XP 749337.1). (C) Alignment in ClustalW of the amino acid sequences of glutamine synthetase II from E. huxleyi (TPA BK005974), the pelagophyte A. anophagefferens (gw1.2.832.1|Auran1), the diatom P. tricornutum (Phatr1:14218), the chlorophyte C. reinhardtii (Q42688), and the vascular plant A. thaliana (S18602).

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