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GAMETOGENESIS AND GAMETE RELEASE OF ULVA MUTABILIS AND ULVA LACTUCA (CHLOROPHYTA): REGULATORY EFFECTS AND CHEMICAL CHARACTERIZATION OF THE “SWARMING INHIBITOR”1
Article first published online: 10 MAR 2010
© 2010 Phycological Society of America
Journal of Phycology
Volume 46, Issue 2, pages 248–259, April 2010
How to Cite
Wichard, T. and Oertel, W. (2010), GAMETOGENESIS AND GAMETE RELEASE OF ULVA MUTABILIS AND ULVA LACTUCA (CHLOROPHYTA): REGULATORY EFFECTS AND CHEMICAL CHARACTERIZATION OF THE “SWARMING INHIBITOR”. Journal of Phycology, 46: 248–259. doi: 10.1111/j.1529-8817.2010.00816.x
Received 15 October 2008. Accepted 12 October 2009.
- Issue published online: 31 MAR 2010
- Article first published online: 10 MAR 2010
Appendix S1. Methods, results, and the following figures:
Figure S1. Phenotypic changes of blade cells during gametogenesis and gamete release. A gametophyte of Ulva mutabilis [wt-G mt(-)] grown under standard conditions was induced for gametogenesis.
Figure S2. UV spectrum and relation of the absorbance to the biological activity. The absorbance spectrum of the SWI from Ulva lactuca was measured at a concentration of 104 units &#U2219; mL-1 (a). The absorbance at 233 nm of four independently isolated and maximally purified U. lactuca-SWI preparations was measured in relation to the SWI activity determined by the SWI standard assay (b).
Figure S3. Mass spectra of SWI from Ulva lactuca and Ulva mutabilis. The ESI mass spectrum of SWI from U. lactuca purified to homogeneity (Fig. 6). Five microliters of SWI solution was directly injected into the mass spectrometer [5,000 units in 50% acetonitrile (v/v); 0.05% TFA]. For measuring the ESI mass spectrum of SWI from U. mutabilis, a crude preparation from the SepPac C18 cartridge was used. The mass and UV spectrum of the SWI activity peak was measured inline to the HPLC column, during elution with a linear gradient of aqueous acetonitrile [0–75% v/v; 0.05% acetic acid (pH 4.0)].
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