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Figure S1. FTIR spectra of the highly sulfated extract V1. Comparison with the nonsulfated mannan-rich RW2 residue is included in order to identify diagnostic absorbance bands exclusive for sulfate groups. FTIR, Fourier transform infrared; RW2, residue obtained after hot-water extractions.

Figure S2. Immunodot-blot screening of HRGP-L epitopes (AGPs and extensins) on cell-wall fractions V1 and V2 isolated from Codium vermilara (see also Table S1 in the supplementary material). Only positive labeling of antibodies against HRGP-L epitopes present in V1-V2 were included in the figure. AGPs, arabinogalactan proteins; HRGP-L, hydroxyproline-rich glycoprotein-like.

Figure S3. Proposed cell-wall model at the utricle tip (named as mucron) for Codium fragile.

Table S1. Mannan and sulfated polymer contents in the cell walls of Codium vermilara.

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