Received 15 August 2009. Accepted 28 January 2010.
CONSTRUCTION OF A GENETIC LINKAGE MAP FOR PORPHYRA HAITANENSIS (BANGIALES, RHODOPHYTA) BASED ON SEQUENCE-RELATED AMPLIFIED POLYMORPHISM AND SIMPLE SEQUENCE REPEAT MARKERS1
Article first published online: 21 JUN 2010
© 2010 Phycological Society of America
Journal of Phycology
Volume 46, Issue 4, pages 780–787, August 2010
How to Cite
Chaotian, X., Changsheng, C., Yan, X. and Dehua, J. (2010), CONSTRUCTION OF A GENETIC LINKAGE MAP FOR PORPHYRA HAITANENSIS (BANGIALES, RHODOPHYTA) BASED ON SEQUENCE-RELATED AMPLIFIED POLYMORPHISM AND SIMPLE SEQUENCE REPEAT MARKERS. Journal of Phycology, 46: 780–787. doi: 10.1111/j.1529-8817.2010.00855.x
- Issue published online: 2 AUG 2010
- Article first published online: 21 JUN 2010
- DH population;
- genetic linkage map;
- Porphyra haitanensis;
Molecular markers and molecular genetic maps are prerequisites for molecular breeding in any plant species. A comprehensive genetic linkage map for cultivated Porphyra haitanensis T. J. Chang et B. F. Zheng has not yet been developed. In this study, 157 double haploid (DH) lines [derived from a YSIII (wildtype) × RTPM (red-type artificial pigmentation mutant) cross] were used as a mapping population in P. haitanensis. A total of 60 pairs of sequence-related amplified polymorphism (SRAP) primers and 39 pairs of simple sequence repeat (SSR) primers were used to detect polymorphisms between the two parents. Fifteen SRAP and 16 SSR polymorphic primer pairs were selected to analyze the DH population. A linkage genetic map comprising 67 SRAP markers and 20 SSR markers in five linkage groups, with a total length of 830.6 cM and an average of 10.13 cM between markers, was constructed. The markers were distributed evenly in all linkage groups without clustering. The linkage groups comprised 12–23 markers ranging in length from 134.2 to 197.3 cM. The estimated genome length of P. haitanensis was 942.4 cM, with 88.1% coverage. This is the first report of a comprehensive genetic map in P. haitanensis. The map presented here will provide a basis for the development of high-density genetic linkage maps and lay the foundation for molecular breeding work in P. haitanensis.