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SEQUENCE AND STRUCTURAL ANALYSIS OF κ-CARRAGEENAN-DERIVED OLIGOSACCHARIDES BY TWO-DIMENSIONAL NUCLEAR MAGNETIC RESONANCE1
Version of Record online: 9 JUL 2010
© 2010 Phycological Society of America
Journal of Phycology
Volume 46, Issue 4, pages 831–838, August 2010
How to Cite
Zhang, W., Liu, M., Hansen, P. E., Yu, G., Yang, B. and Zhao, X. (2010), SEQUENCE AND STRUCTURAL ANALYSIS OF κ-CARRAGEENAN-DERIVED OLIGOSACCHARIDES BY TWO-DIMENSIONAL NUCLEAR MAGNETIC RESONANCE. Journal of Phycology, 46: 831–838. doi: 10.1111/j.1529-8817.2010.00861.x
Received 10 March 2009. Accepted 28 January 2009.
- Issue online: 2 AUG 2010
- Version of Record online: 9 JUL 2010
- nuclear magnetic resonance;
κ-Carrageenan was hydrolyzed with mild hydrochloric acid and separated into a series of oligosaccharides, the sequences and structures of which were investigated by double-quantum filtered correlation spectroscopy (DQF-COSY), total correlation spectroscopy (TOCSY), heteronuclear multiple-quantum coherence (HMQC), and heteronuclear multiple-bond correlation (HMBC) techniques, respectively. The chemical structures and conformations of the individual sugar residues were identified, as well as the sequential connectivity of the oligosaccharides. The interresidue nuclear Overhauser effects (NOEs)/rotating frame Overhauser effects (ROEs) revealed an ordered helical structure of the carrageenan oligosaccharide chains. Therefore, a general two-dimensional (2-D) NMR methodology for the unambiguous sequence and structure analysis of κ-carrageenan-derived oligosaccharides was established in this study.