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INTRACELLULAR LOCALIZATION OF AN ENDOGENOUS CELLULOSE SYNTHASE OF MICRASTERIAS DENTICULATA (DESMIDIALES, CHLOROPHYTA) BY MEANS OF TRANSIENT GENETIC TRANSFORMATION1
Article first published online: 20 JUL 2010
© 2010 Phycological Society of America
Journal of Phycology
Volume 46, Issue 4, pages 839–845, August 2010
How to Cite
Vannerum, K., Abe, J., Sekimoto, H., Inzé, D. and Vyverman, W. (2010), INTRACELLULAR LOCALIZATION OF AN ENDOGENOUS CELLULOSE SYNTHASE OF MICRASTERIAS DENTICULATA (DESMIDIALES, CHLOROPHYTA) BY MEANS OF TRANSIENT GENETIC TRANSFORMATION. Journal of Phycology, 46: 839–845. doi: 10.1111/j.1529-8817.2010.00867.x
Received 30 July 2009. Accepted 12 March 2010.
- Issue published online: 2 AUG 2010
- Article first published online: 20 JUL 2010
Figure S1. Intracellular localization of cgfp in Micrasterias denticulata following particle bombardment with pSA405A. Individual confocal GFP fluorescence z-stack images, 2.29 μm apart to cover the cell depth. The free cgfp is located inside the nucleus and in the cytoplasm. Scale bar, 50 μm. GFP, green fluorescent protein.
Figure S2. Full-length deduced amino acid sequence of M. denticulata CesA1 (Gen Bank accession number GU808357) aligned with deduced amino acid sequences of CesAs from Mesotaenium caldariorum (accession number: AF25360), Physcomitrella patens subsp, patens (accession number: XP_001767133), and Populus tremuloides (accession number: AY 196961). Asterisks indicate identical residues; colons and periods indicate full conservation of strong and weak groups, respectively (Larkin et al. 2007). The zinc-binding domain is indicated below the sequences with an unshaded box, the cellulose synthase protein family domain (PF03552) with gray shading.
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