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BIODEGRADATION OF PHTHALATE ESTERS BY CYANOBACTERIA1
Article first published online: 27 SEP 2010
© 2010 Phycological Society of America
Journal of Phycology
Volume 46, Issue 6, pages 1106–1113, December 2010
How to Cite
Babu, B. and Wu, J.-T. (2010), BIODEGRADATION OF PHTHALATE ESTERS BY CYANOBACTERIA. Journal of Phycology, 46: 1106–1113. doi: 10.1111/j.1529-8817.2010.00896.x
Received 17 September 2009. Accepted 19 April 2010.
- Issue published online: 1 DEC 2010
- Article first published online: 27 SEP 2010
- phthalate ester
Phthalate esters (PEs) are endocrine-disrupting pollutants that are ubiquitous in the environment and can be degraded by microorganisms. In this study, we investigated the kinetics and pathway of biodegradation of di-n-butyl phthalate (DBP), diethyl phthalate (DEP), and dimethyl phthalate (DMP) by cyanobacteria Anabaena flos-aquae G. S. West (strain 4054) and two strains of Microcystis aeruginosa (Kütz.) Kütz. (strain 2396 and strain SM). Gas chromatography/mass spectroscopy (GC/MS) and a deuterium-labeled compound were used to analyze the degrading intermediates. The findings revealed that all three organisms were capable of metabolizing PE, and that among these organisms, A. flos-aquae achieved the highest degradation. Additionally, the biodegradation of DBP, DEP, and DMP followed first-order kinetics. Moreover, the results of the enzymatic study suggested that PE was degraded through transesterification on the side chains rather than deesterification. Finally, experiments using deuterium-labeled DBP showed that there were two degradation pathways: C16 C14 C12 C10 C8 and C16 C15 C13 C11 C9. Based on our results, the biodegradation pathway of PE for cyanobacteria was suggested.