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A ONE-SHOT SOLUTION TO BACTERIAL AND FUNGAL CONTAMINATION IN THE GREEN ALGA CHLAMYDOMONAS REINHARDTII CULTURE BY USING AN ANTIBIOTIC COCKTAIL1
Article first published online: 15 OCT 2010
© 2010 Phycological Society of America
Journal of Phycology
Volume 46, Issue 6, pages 1356–1358, December 2010
How to Cite
Kan, Y. and Pan, J. (2010), A ONE-SHOT SOLUTION TO BACTERIAL AND FUNGAL CONTAMINATION IN THE GREEN ALGA CHLAMYDOMONAS REINHARDTII CULTURE BY USING AN ANTIBIOTIC COCKTAIL. Journal of Phycology, 46: 1356–1358. doi: 10.1111/j.1529-8817.2010.00904.x
Received 09 November 2009. Accepted 20 July 2010.
- Issue published online: 1 DEC 2010
- Article first published online: 15 OCT 2010
- bacterial contamination;
- fungal contamination;
Keeping sterile stocks or cultures of microalgae is fundamental to microalgae biotechnology as well as basic scientific research. However, contamination by bacteria and/or fungi in microalgae cultures or stocks is often a problem. Here, we have developed a strategy for reducing or eliminating bacterial and fungal contamination by using a cocktail of antibiotics. Chlamydomonas reinhardtii P. A. Dang., a widely used unicellular green alga, has been used as a testing organism. A combination of ampicillin, cefotaxime, and carbendazim removed or reduced contamination by three different bacteria and two different fungi tested. A step-by-step procedure is provided, which is simple, economical, and effective.