Present address: Graduate School of Environmental and Life Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8572, Japan.
EVOLUTIONARY ANALYSES OF THE NUCLEAR-ENCODED PHOTOSYNTHETIC GENE psbO FROM TERTIARY PLASTID-CONTAINING ALGAE IN DINOPHYTA1
Article first published online: 30 MAR 2011
© 2011 Phycological Society of America
Journal of Phycology
Volume 47, Issue 2, pages 407–414, April 2011
How to Cite
Yokoyama, A., Takahashi, F., Kataoka, H., Hara, Y. and Nozaki, H. (2011), EVOLUTIONARY ANALYSES OF THE NUCLEAR-ENCODED PHOTOSYNTHETIC GENE psbO FROM TERTIARY PLASTID-CONTAINING ALGAE IN DINOPHYTA. Journal of Phycology, 47: 407–414. doi: 10.1111/j.1529-8817.2011.00961.x
Received 19 February 2010. Accepted 13 January 2011.
- Issue published online: 4 APR 2011
- Article first published online: 30 MAR 2011
- N-terminal region;
- psbO genes
Although the dinophytes generally possess red-algal-derived secondary plastids, tertiary plastids originating from haptophyte and diatom ancestors are recognized in some lineages within the Dinophyta. However, little is known about the nuclear-encoded genes of plastid-targeted proteins from the dinophytes with diatom-derived tertiary plastids. We analyzed the sequences of the nuclear psbO gene encoding oxygen-evolving enhancer protein from various algae with red-algal-derived secondary and tertiary plastids. Based on our sequencing of 10 new genes and phylogenetic analysis of PsbO amino acid sequences from a wide taxon sampling of red algae and organisms with red-algal-derived plastids, dinophytes form three separate lineages: one composed of peridinin-containing species with secondary plastids, and the other two having haptophyte- or diatom-derived tertiary plastids and forming a robust monophyletic group with haptophytes and diatoms, respectively. Comparison of the N-terminal sequences of PsbO proteins suggests that psbO genes from a dinophyte with diatom-derived tertiary plastids (Kryptoperidinium) encode proteins that are targeted to the diatom plastid from the endosymbiotic diatom nucleus as in the secondary phototrophs, whereas the fucoxanthin-containing dinophytes (Karenia and Karlodinium) have evolved an additional system of psbO genes for targeting the PsbO proteins to their haptophyte-derived tertiary plastids from the host dinophyte nuclei.