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Fig. S1. Evolutionary relationships among GSII enzymes, partial representation of the complete tree.

Fig. S2. Evolutionary relationships among GSII enzymes, continued from Figure S1.Nodes with BBP support > 0.95 are represented by thick lines. RAxML bootstrap values are indicated for nodes recovered in both analyses.

Fig. S3. Bipartite chloroplast transit sequences found chromalveloate GSII sequences.

Fig. S4. Evolutionary relationships among GSIII enzymes, partial representation of the complete tree continued on Figure S5.

Fig. S5. Evolutionary relationships among GSIII enzymes, partial representation of the complete tree continued from Figure S4.

Fig. S6. Mitochondrial target peptides were predicted in silico for GSIII protein sequences from select chromalveolates.

Table S1. Summary of the red algal taxa used in this study. Species names (Taxa), class, strain number (SAG#), and media used for culturing are presented. All cultures were obtained from Sammlung von Algenkulturen and the instructions for media preparation are provided at http://www.epsag.uni-goettingen.de.

Table S2. Degenerate primer sequences used for initial PCR amplification of GSII genes from red algae. Primer sequences are presented using IUBMB single letter codes where I represents inosine.

Table S3. Degenerate primer pairs used for initial PCR amplification of GSII sequences from red algae. Species names (taxa), the initial template used for amplification (genomic DNA or cDNA), and primers used in PCR amplification are provided. Primer sequences are provided in Table S2.

Table S4. Information for GSII protein sequences from organisms used in the present study. GenBank accession numbers and JGI DOE scaffold and protein ID information for the GSII proteins are provided.

Table S5. Information for GSIII protein sequences from organisms used in the present study. GenBank accession numbers and JGI DOE scaffold and protein ID information for the GSIII proteins are provided.

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