Present address: Department Biologie, Systematische Botanik und Mykologie, Ludwig-Maximilians-Universität München, Menzinger Str. 67, D – 80638 München, Germany.
SAME BUT DIFFERENT: TWO NOVEL BICARINATE SPECIES OF EXTANT CALCAREOUS DINOPHYTES (THORACOSPHAERACEAE, PERIDINIALES) FROM THE MEDITERRANEAN SEA1
Article first published online: 21 JUN 2012
© 2012 Phycological Society of America
Journal of Phycology
Volume 48, Issue 5, pages 1107–1118, October 2012
How to Cite
Zinssmeister, C., Soehner, S., Kirsch, M., Facher, E., Sebastian Meier, K. J., Keupp, H. and Gottschling, a. M. (2012), SAME BUT DIFFERENT: TWO NOVEL BICARINATE SPECIES OF EXTANT CALCAREOUS DINOPHYTES (THORACOSPHAERACEAE, PERIDINIALES) FROM THE MEDITERRANEAN SEA. Journal of Phycology, 48: 1107–1118. doi: 10.1111/j.1529-8817.2012.01182.x
Received 3 August 2011. Accepted 13 March 2012.
- Issue published online: 4 OCT 2012
- Article first published online: 21 JUN 2012
- Accepted manuscript online: 4 MAY 2012 09:05PM EST
- coccoid cell;
- cytochrome b;
- molecular systematics;
- ribosomal RNA;
- thecate cell
The diversity of extant calcareous dinophytes (Thoracosphaeraceae, Dinophyceae) is currently not sufficiently recorded. The majority of their coccoid stages are cryptotabulate or entirely atabulate, whereas relatively few forms exhibit at least some degree of tabulation more than the archeopyle. A survey of coastal surface sediment samples from the Mediterranean Sea resulted in the isolation and cultivation of several strains of calcareous dinophytes showing a prominent tabulation. We investigated the morphologies of the thecate and the coccoid cells and conducted phylogenetic analyses using Maximum Likelihood and Bayesian approaches. The coccoid cells showed a distinct reflection of the cingulum (and were thus cingulotabulate), whereas thecal morphology corresponded to the widely distributed and species-rich Scrippsiella. As inferred from molecular sequence data (including 81 new GenBank entries), the strains belonged to the Scrippsiella sensu lato clade of the Thoracosphaeraceae and represented two distinct species. Morphological details likewise indicated two distinct species with previously unknown coccoid cells that we describe here as new, namely S. bicarinata spec. nov. and S. kirschiae spec. nov. Cingulotabulation results from the fusion of processes representing the pre- and postcingular plate series in S. bicarinata, whereas the ridges represent sutures between the cingulum and the pre- and postcingular series in S. kirschiae, respectively. Bicarinate cingulotabulation appears homoplasious among calcareous dinophytes, which is further supported by a comparison to similar, but only distantly related fossil forms.