These authors contributed equally to this work.
SELECTION OF INTERNAL CONTROL GENE FOR EXPRESSION STUDIES IN PORPHYRA HAITANENSIS (RHODOPHYTA) AT DIFFERENT LIFE-HISTORY STAGES1
Version of Record online: 31 MAY 2012
© 2012 Phycological Society of America
Journal of Phycology
Volume 48, Issue 4, pages 1040–1044, August 2012
How to Cite
Wu, X., Niu, J., Huang, A., Xu, M. and Wang, G. (2012), SELECTION OF INTERNAL CONTROL GENE FOR EXPRESSION STUDIES IN PORPHYRA HAITANENSIS (RHODOPHYTA) AT DIFFERENT LIFE-HISTORY STAGES. Journal of Phycology, 48: 1040–1044. doi: 10.1111/j.1529-8817.2012.01188.x
Received 9 October 2011. Accepted 16 February 2012.
- Issue online: 1 AUG 2012
- Version of Record online: 31 MAY 2012
- Accepted manuscript online: 7 MAY 2012 11:43AM EST
- absolute quantification;
- internal control gene;
- life-history stage;
- Porphyra haitanensis;
Accurate gene quantification depends on the use of an appropriate internal control gene, which should be verified before its use for normalizing data. Housekeeping genes, which are expressed at relatively constant levels, are generally regarded as candidate internal control genes. To determine the ideal internal control for gene expression profiles for Porphyra haitanensis T. J. Chang et B. F. Zheng (Bangiales, Rhodophyta) at different life-history stages, we used absolute quantification to assess the expression levels of six housekeeping genes (18S ribosomal RNA, 30S ribosomal protein, glyceraldehyde-3-phosphate dehydrogenase, elongation factor 3, alpha-tubulin, and beta-tubulin) at the sporophyte and gametophyte stages. Housekeeping genes were selected by comparing the differences of observed copy numbers in sporophytes and in gametophytes. TubB (beta-tubulin) was found to be the optimal internal control gene, because it showed the smallest difference of gene expression. Compared with TubB, other housekeeping genes had greater variation of expression to different degrees.