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Fig.  S1. Gel electrophoresis of total RNA extracted sporophytes and gametophytes. The DNA marker bands from top to bottom were 2,000, 1,000, 750, 500, 250 and 100 bp.

Fig.   S2. The standard curves constructed for 18S (a), EF3 (b), GAPDH (c), RPS (d), TubA (e), TubB (f). The results showed that amplification efficiency was between 96% and 103%, and linear correlation coefficient was >0.99.

Fig.  S3. The melting curve analysis for 18S (a), EF3 (b), GAPDH (c), RPS (d), TubA (e), TubB (f). Melting peaks were examined with standard samples and unknown samples (sporophytes and gametophytes). The melting curve for each gene had only one peak.

Table  S1. Primer sequences and amplicon size of candidate housekeeping genes.

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FilenameFormatSizeDescription
JPY_1188_sm_FigS1.tif100KSupporting info item
JPY_1188_sm_FigS2.tif477KSupporting info item
JPY_1188_sm_FigS3.tif627KSupporting info item
JPY_1188_sm_TableS1.doc29KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.