Present address: Bamfield Marine Sciences Centre, 100 Pachena Rd., Bamfield, British Columbia V0R 1B0, Canada
A SURVEY OF BANGIALES (RHODOPHYTA) BASED ON MULTIPLE MOLECULAR MARKERS REVEALS CRYPTIC DIVERSITY1
Article first published online: 18 JUN 2012
© 2012 Phycological Society of America
Journal of Phycology
Volume 48, Issue 4, pages 869–882, August 2012
How to Cite
Kucera, H. and Saunders, G. W. (2012), A SURVEY OF BANGIALES (RHODOPHYTA) BASED ON MULTIPLE MOLECULAR MARKERS REVEALS CRYPTIC DIVERSITY. Journal of Phycology, 48: 869–882. doi: 10.1111/j.1529-8817.2012.01193.x
Received 1 October 2010. Accepted 19 January 2012.
- Issue published online: 1 AUG 2012
- Article first published online: 18 JUN 2012
- Accepted manuscript online: 17 MAY 2012 12:55PM EST
- DNA barcoding;
The Bangiales is a diverse order consisting of 28 species in Canada. Morphological simplicity and similarity among species has led to taxonomic confusion and the need for molecular techniques for species identification. This study is the first to employ the standardized DNA barcode marker COI-5P in a broad floristic survey of the Bangiales in Canadian marine waters. A total of 37 species were ultimately sequenced, 29 of which occurred in Canada. Molecular results led to the synonymization of Wildemania cuneiformis with W. amplissima, as well as the description of two new species: Porphyra corallicola sp. nov. and Pyropia peggicovensis sp. nov., and discovery of another five putative new species. Comparison of the performance of COI-5P as a species identification tool relative to rbcL (large subunit of ribulose-1,5-bisphosphate carboxylase oxygenase) and the UPA (universal plastid amplicon) revealed that, although each marker had strengths and weaknesses, the COI-5P showed the highest species-discriminatory power due to its high level of interspecific variation. The rbcL was further used to place the new species into a phylogenetic context, whereas UPA was not recommended for species identification in the Bangiales owing to within-individual heterogeneity between the two copies present in the plastid genomes in some lineages.