THE ROLE OF ZINC FINGER PROTEIN IN RNAi INTERFERENCE IN A UNICELLULAR GREEN ALGA CHLAMYDOMONAS REINHARDTII (CHLOROPHYCEAE)
Version of Record online: 27 AUG 2012
© 2012 Phycological Society of America
Journal of Phycology
Volume 48, Issue 5, pages 1299–1303, October 2012
How to Cite
Uchida, H., Ikeuchi, E., Yamasaki, T. and Ohama, T. (2012), THE ROLE OF ZINC FINGER PROTEIN IN RNAi INTERFERENCE IN A UNICELLULAR GREEN ALGA CHLAMYDOMONAS REINHARDTII (CHLOROPHYCEAE). Journal of Phycology, 48: 1299–1303. doi: 10.1111/j.1529-8817.2012.01214.x
- Issue online: 4 OCT 2012
- Version of Record online: 27 AUG 2012
- Accepted manuscript online: 18 JUL 2012 07:36AM EST
- Manuscript Accepted: 6 APR 2012
- Manuscript Received: 15 SEP 2011
- Japan Securities Scholarship Foundation
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|jpy1214-sup-0001-FigS1.pdf||application/PDF||1106K||Fig. S1. Uniform size of colonies raised from a stable RNAi-37 subclone on the spectinomycin-containing plate. The strain of RNAi-37 subclone that has a stable RNAi level was obtained by successive selection of a smaller colony on the spectinomycin-containing agar plate (50 μg · mL−1).|
|jpy1214-sup-0002-FigS2.pdf||application/PDF||1227K||Fig. S2. Detection of zinc finger motif-protein gene transcript in the NX-transformants. RT-PCR was performed for two lines of the transformants that bear Au5.g2189 as a transgene. Specific primers for zinc finger motif-protein cDNA were used as in the experiment shown in Fig. 2. Note that wild-type CC-124 and 92-12C1 were used as positive and negative controls, respectively. As an internal control, the ribosomal protein 3 (rp3) cDNA was used.|
|jpy1214-sup-0003-FigS3.pdf||application/PDF||87K||Fig. S3. Semiquantitative RT-PCR was performed to estimate the relative amount of the remaining aadA mRNA in various transformants. The amount of transcripts was determined using densitometry of PCR products on the ethidium bromide-stained gel using Image J software. Experiments were repeated five times and values are presented as mean ± standard deviation.|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.