Diethyl Ether Inhibits Ethanol Metabolism in Vivo by Interaction with Alcohol Dehydrogenase
Version of Record online: 11 APR 2006
Alcoholism: Clinical and Experimental Research
Volume 11, Issue 2, pages 163–166, April 1987
How to Cite
Normann, P. T., Ripel, Å. and Mørland, J. (1987), Diethyl Ether Inhibits Ethanol Metabolism in Vivo by Interaction with Alcohol Dehydrogenase. Alcoholism: Clinical and Experimental Research, 11: 163–166. doi: 10.1111/j.1530-0277.1987.tb01282.x
- Issue online: 11 APR 2006
- Version of Record online: 11 APR 2006
- Received for publication May 27, 1986, revised manuscript received July 29, 1986, accepted August 5, 1986
The ethanol disappearance rate was determined in fed rats given 20-40 mM ethanol and anesthetized with pentobarbital (control group) and diethyl ether. The control ethanol disappearance rate was 0.27 ± 0.02 mM/min (±SD, n= 4). Rats anesthetized with diethyl ether (blood levels of 9–13 mM) revealed an ethanol disappearance rate of 0.13 ± 0.05 mM/min (±SD, n= 7), i.e., 52% inhibition of the control rate.
Kinetic studies on crystalline and lyophilized alcohol dehydrogenase from equine liver demonstrated that 20 mM diethyl ether inhibited the initial rate of ethanol oxidation by 55%. By using ethanol as the variable substrate the inhibition of alcohol dehydrogenase was described by a mixed noncompetitive/uncompetitive mechanism.