Nucleotide Sequence of the ADH23 Gene Encoding the Human Alcohol Dehydrogenase β3 Subunit

Authors


  • Supported by U.S. Public Health Service Grants R01-AA06460, R01-AA02342, and T32-AA07462. The Bionet National Computer Resource was supported by USPHS Grant U41-RRO1685.

Reprint requests: Dr. Howard J. Edenberg, Department of Biochemistry, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46223.

Abstract

All nine exons of the ADH23 allele, which encodes the human alcohol dehydrogenase β3 subunit, have been cloned and sequenced. Comparison of this sequence to the ADH21 nucleotide sequence revealed only a single difference that results in an amino acid change, thus proving that the significant kinetic differences between these two isozymes is due to the Cys for Arg substitution at position 369. There are also two silent polymorphisms, and two changes in noncoding regions.

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