Varying Effects of Ethanol on Transfected Cell Lines

Authors


  • This work was supported in part by Grant IRPG R01AA10030 from the National Institute on Alcohol Abuse and Alcoholism.

Reprint requests: Adrienne S. Gordon, Ph.D., Ernest Gallo Clinic and Research Center, 1001 Potrero Avenue, Building 1, #101, San Francisco, CA 94110.

Abstract

The use of transfected cell lines has provided a powerful approach to study the functions of transfected proteins. This approach has also proven useful to determine the effects of acute and chronic ethanol exposure on particular proteins. We show here, however, that the effects of ethanol on transfected proteins vary between transiently transfected cells and stably transfected cell lines. Moreover, we found that the effect of ethanol on a particular protein depends on the plasmid vectors used for the transfection.

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