This work was supported in part by Grant IRPG R01AA10030 from the National Institute on Alcohol Abuse and Alcoholism.
Varying Effects of Ethanol on Transfected Cell Lines
Article first published online: 30 MAY 2006
Alcoholism: Clinical and Experimental Research
Volume 22, Issue 1, pages 163–166, February 1998
How to Cite
Asai, K., Ishii, A., Yao, U., Diamond, I. and Gordon, A. S. (1998), Varying Effects of Ethanol on Transfected Cell Lines. Alcoholism: Clinical and Experimental Research, 22: 163–166. doi: 10.1111/j.1530-0277.1998.tb03633.x
- Issue published online: 30 MAY 2006
- Article first published online: 30 MAY 2006
- Received for publication May 15, 1997; accepted August 26, 1997
- NG108-15 Cells;
- CAT Expression;
The use of transfected cell lines has provided a powerful approach to study the functions of transfected proteins. This approach has also proven useful to determine the effects of acute and chronic ethanol exposure on particular proteins. We show here, however, that the effects of ethanol on transfected proteins vary between transiently transfected cells and stably transfected cell lines. Moreover, we found that the effect of ethanol on a particular protein depends on the plasmid vectors used for the transfection.