Binge Ethanol Exposure in Adult Rats Causes Necrotic Cell Death

Authors

  • J. A. Obernier,

    1. Bowles Center for Alcohol Studies (JAO, FTC); Departments of Pharmacology (JAO, FTC), Pathology (TWB), and Psychiatry (FTC), University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
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  • T. W. Bouldin,

    1. Bowles Center for Alcohol Studies (JAO, FTC); Departments of Pharmacology (JAO, FTC), Pathology (TWB), and Psychiatry (FTC), University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
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  • F. T. Crews

    Corresponding author
    1. Bowles Center for Alcohol Studies (JAO, FTC); Departments of Pharmacology (JAO, FTC), Pathology (TWB), and Psychiatry (FTC), University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
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  • A portion of this work was previously reported in abstract form in Society for Neuroscience Abstracts (2000) 26:1566.

  • This research was supported by the National Institute of Alcohol Abuse and Alcoholism.

Fulton T. Crews, PhD, Bowles Center for Alcohol Studies, CB#7178, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7178; Fax: 919-966-9099; E-mail: ftcrews@med.unc.edu

Abstract

Background: Although alcoholics show neurodegeneration after decades of drinking, recent studies with an animal model of binge drinking have found corticolimbic damage after as few as four days. Neurodegeneration can occur through apoptotic or necrotic mechanisms. The goal of this research is to characterize the time course of binge ethanol-induced neurodegeneration and to identify apoptotic or necrotic characteristics of this neurodegeneration.

Methods: Histologic methods (e.g., amino cupric silver staining, Fluoro-Jade B, hematoxylin and eosin, transmission electron microscopy) were used to quantify the time course of degeneration and to characterize the ultrastructural changes that occur with binge ethanol-induced neurodegeneration.

Results: After 2 days of binge ethanol, significant damage was evident in the olfactory bulb. After 4 days of binge ethanol, there was significant damage in the agranular insular cortex, anterior piriform cortex, perirhinal cortex, lateral entorhinal cortex, and the temporal dentate gyrus. Ultrastructural examination revealed shrunken soma, vacuolated cytoplasm, pyknotic nucleus, and irregularly clumped chromatin consistent with dark cell degeneration, a form of necrotic neuronal death.

Conclusions: Binge drinking causes necrotic neurodegeneration after 2 days of exposure and increased damage after 4 days but does not increase during withdrawal. These studies indicate that binge drinking induced neurodegeneration is necrotic and occurs during ethanol intoxication and not as a result of ethanol withdrawal.

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