Supported by Grant AA14874 from the National Institute on Alcohol Abuse and Alcoholism.
Ethanol Preference Is Inversely Correlated With Ethanol-Induced Dopamine Release in 2 Substrains of C57BL/6 Mice
Article first published online: 25 JUL 2007
Alcoholism: Clinical and Experimental Research
Volume 31, Issue 10, pages 1669–1676, October 2007
How to Cite
Ramachandra, V., Phuc, S., Franco, A. C. and Gonzales, R. A. (2007), Ethanol Preference Is Inversely Correlated With Ethanol-Induced Dopamine Release in 2 Substrains of C57BL/6 Mice. Alcoholism: Clinical and Experimental Research, 31: 1669–1676. doi: 10.1111/j.1530-0277.2007.00463.x
- Issue published online: 30 JUL 2007
- Article first published online: 25 JUL 2007
- Received for publication April 23, 2007; accepted May 25, 2007.
- Ventral Striatum;
- C57BL/6 Mice;
Background: The C57BL/6 mouse model has been used extensively in alcohol drinking studies, yet significant differences in ethanol preference between substrains exist. Differences in ethanol-induced dopamine release in the ventral striatum could contribute to this variability in drinking behavior as dopamine has been implicated in the reinforcing properties of ethanol.
Methods: A 2-bottle choice experiment investigated the difference in ethanol preference between C57BL/6J and C57BL/6NCrl animals. Microdialysis was used to determine dopamine release and ethanol clearance in these 2 substrains after intraperitoneal injections of 1.0, 2.0 and 3.0 g/kg ethanol or saline.
Results: C57BL/6J mice exhibited significantly greater ethanol preference and less ethanol-stimulated dopamine release compared with C57BL/6NCrl mice. The intraperitoneal injections of ethanol caused a significant increase in dopamine in both substrains at all 3 doses with significant differences between substrains at the 2 highest alcohol doses. Saline injections had a significant effect on dopamine release when given in a volume equivalent to the 3 g/kg ethanol dose. Ethanol pharmacokinetics were similar in the 2 substrains at all 3 doses.
Conclusions: Ethanol-induced dopamine release in the ventral striatum may contribute to the differences in alcohol preference between C57BL/6J and C57BL/6NCrl mice.