This study was supported by an NIH/NIAAA grant (#1R01AA015325-01A2) to RG.
Differences in Acute Alcohol-Induced Behavioral Responses Among Zebrafish Populations
Article first published online: 24 JUL 2008
Copyright © 2008 by the Research Society on Alcoholism
Alcoholism: Clinical and Experimental Research
Volume 32, Issue 10, pages 1763–1773, October 2008
How to Cite
Gerlai, R., Ahmad, F. and Prajapati, S. (2008), Differences in Acute Alcohol-Induced Behavioral Responses Among Zebrafish Populations. Alcoholism: Clinical and Experimental Research, 32: 1763–1773. doi: 10.1111/j.1530-0277.2008.00761.x
- Issue published online: 17 SEP 2008
- Article first published online: 24 JUL 2008
- Received for publication January 11, 2008; accepted May 22, 2008.
- Alcohol Abuse;
- Acute Alcohol Administration;
- Strain Comparison;
- Zebra Danio
Background: With the arsenal of genetic tools available for zebrafish, this species has been successfully used to investigate the genetic aspects of human diseases from developmental disorders to cancer. Interest in the behavior and brain function of zebrafish is also increasing as CNS disorders may be modeled and studied with this species. Alcoholism and alcohol abuse are among the most devastating and costliest diseases. However, the mechanisms of these diseases are not fully understood. Zebrafish has been proposed as a model organism to study such mechanisms. Characterization of alcohol’s effects on zebrafish is a necessary step in this research.
Methods: Here, we compare the effects of acute alcohol (EtOH) administration on the behavior of zebrafish from 4 distinct laboratory-bred populations using automated as well as observation based behavioral quantification methods.
Results: Alcohol treatment resulted in significant dose-dependent behavioral changes but the dose–response trajectories differed among zebrafish populations.
Conclusions: The results demonstrate for the first time a genetic component in alcohol responses in adult zebrafish and also show the feasibility of high throughput behavioral screening. We discuss the exploration and exploitation of the genetic differences found.